[Type text]
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
No. Experiment Pages
0 Safety Rules and Regulations in Chemistry Laboratory 3
0 Laboratory Report Guideline 5
1 Gravimetric Analysis: Determination Of Chloride Ion 7
In Sodium Chloride Salt
2 Extraction Of Caffeine From Beverages 15
3 Standardization Of Sodium Hydroxide And 21
Determination Of Unknown Acid Concentration
4 Determination Of Caffeine In Beverages By 28
High Performance Liquid
Chromatography (Hplc)
5 Determination Of Quinine By 35
Molecular Fluorescence
Spectroscopy
SKA3013 LABORATORY MANUAL
.
ANALYTICAL CHEMISTRY
2
2022/2023
new update
,[Type text]
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
SAFETY RULES AND REGULATIONS IN CHEMISTRY LABORATORY
1. DO NOT ENTER the laboratory without the permission of the laboratory instructor.
2. All bags should be placed on the rack provided. All gadgets (handphone, tablet, etc)
are
NOT ALLOWED during experiments.
3. Please wear a proper attire to work in the lab (Short pants, short skirt,
“purdah”, sleeveless shirt are not permitted).
4. Wearing jewellery is not permitted in the laboratory.
5. Long hair and scarf must be properly manageable.
6. DO NOT wear contact lenses.
7. Wearing personal protection equipment (PPE) is compulsory in laboratory
such as lab coat, cover shoes and safety glasses, gloves and etc.
8. Student are NOT ALLOWED to smoke, drink, eat, play around and run in laboratory
9. Know the location of all safety equipment.
10. No experiment can be performed without the permission of the laboratory instructor.
11. Read the label on the container of a chemical twice to make sure you are
using the right chemicals. Follow the instructions properly. DO NOT waste
chemicals. Use required amount only.
12. DO NOT contaminate chemicals. Once chemical has been removed from their
containers, DO NOT put it back into the stock container.
13. DO NOT use cracked or chipped glassware.
14. NEVER use mouth suction when using a pipette. Use a pipette filler/bulb.
15. DO NOT leave any heating, vigorous or rapid reaction unattended.
16. Always use fume hood when working with flammable organic compounds,
strong acid and base, volatile solvent, corrosive and toxic materials.
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
. 3
,[Type text]
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
17. DO NOT heat flammable materials directly.
18. Make sure your surrounding is safe before using Bunsen burner.
19. While heating chemicals in test tubes, NEVER point the tubes to yourself or others.
20. Waste chemicals must be disposed properly by placing them in waste
container provided. Ask your instructor how to discard used reagents.
21. Broken apparatus, water and gas leakage must be reported to laboratory
assistant on duty immediately.
22. In case on fire or hearing emergency siren, evacuate the laboratory
quickly in orderly manner. In case of small accident or small fire occurs, try
to overcome them wisely.
23. Report any accidents (minor or major) to your laboratory instructor. This
accident must be recorded in log book “CATATAN KEMALANGAN”.
24. Clean all glassware and place them appropriately before leaving laboratory.
Make sure to switch off instruments, water and gas supplies.
25. Final year project students should require permission from supervisor and
head of department if they want to work after normal working hours.
AN EXPERIMENT DONE WELL IS......
AN EXPERIMENT DONE SAFELY
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
. 4
, [Type text]
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
GUIDELINE TO WRITE LAB REPORT
OBJECTIVE
State the objective clearly in a complete sentence. A few explanatory sentences
may be included, if needed.
The objective should answer the question: What is the lab objective designed to determine?
EXPERIMENTAL PROCEDURE
The experimental methods should give a detailed description in your own words of
how YOU accomplished the experimentation (Passive voice sentences).
*Tips: This should include equipment used in the experiment as well as how it was
used. The description should have sufficient detail so that another experimenter
could duplicate your efforts.
Use sketches and diagrams to describe the experimental set-up. Label the main
components.
Tips: Any information copied directly from Lab manuals or other references should be stated
within quotes and referred, otherwise, it is considered plagiarism.
RESULTS AND DISCUSSION
This is the most important part of the report. Summarize your results in the
introductory sentence. Relate your results to your objective. Present the results in
the easiest way for your reader to understand: graphs, tables, figures, etc.
Spreadsheets are often an ideal tool for organizing the data, analyzing the data, and
generating graphs and tables. All tables and figures should be accompanied by
comments or discussions in the text of report; use a numbering system for
identification of each one.
All figures and tables must have numbers and captions. While the table captions
should be placed over the table, figure captions should be placed below the figure.
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
. 5
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
No. Experiment Pages
0 Safety Rules and Regulations in Chemistry Laboratory 3
0 Laboratory Report Guideline 5
1 Gravimetric Analysis: Determination Of Chloride Ion 7
In Sodium Chloride Salt
2 Extraction Of Caffeine From Beverages 15
3 Standardization Of Sodium Hydroxide And 21
Determination Of Unknown Acid Concentration
4 Determination Of Caffeine In Beverages By 28
High Performance Liquid
Chromatography (Hplc)
5 Determination Of Quinine By 35
Molecular Fluorescence
Spectroscopy
SKA3013 LABORATORY MANUAL
.
ANALYTICAL CHEMISTRY
2
2022/2023
new update
,[Type text]
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
SAFETY RULES AND REGULATIONS IN CHEMISTRY LABORATORY
1. DO NOT ENTER the laboratory without the permission of the laboratory instructor.
2. All bags should be placed on the rack provided. All gadgets (handphone, tablet, etc)
are
NOT ALLOWED during experiments.
3. Please wear a proper attire to work in the lab (Short pants, short skirt,
“purdah”, sleeveless shirt are not permitted).
4. Wearing jewellery is not permitted in the laboratory.
5. Long hair and scarf must be properly manageable.
6. DO NOT wear contact lenses.
7. Wearing personal protection equipment (PPE) is compulsory in laboratory
such as lab coat, cover shoes and safety glasses, gloves and etc.
8. Student are NOT ALLOWED to smoke, drink, eat, play around and run in laboratory
9. Know the location of all safety equipment.
10. No experiment can be performed without the permission of the laboratory instructor.
11. Read the label on the container of a chemical twice to make sure you are
using the right chemicals. Follow the instructions properly. DO NOT waste
chemicals. Use required amount only.
12. DO NOT contaminate chemicals. Once chemical has been removed from their
containers, DO NOT put it back into the stock container.
13. DO NOT use cracked or chipped glassware.
14. NEVER use mouth suction when using a pipette. Use a pipette filler/bulb.
15. DO NOT leave any heating, vigorous or rapid reaction unattended.
16. Always use fume hood when working with flammable organic compounds,
strong acid and base, volatile solvent, corrosive and toxic materials.
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
. 3
,[Type text]
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
17. DO NOT heat flammable materials directly.
18. Make sure your surrounding is safe before using Bunsen burner.
19. While heating chemicals in test tubes, NEVER point the tubes to yourself or others.
20. Waste chemicals must be disposed properly by placing them in waste
container provided. Ask your instructor how to discard used reagents.
21. Broken apparatus, water and gas leakage must be reported to laboratory
assistant on duty immediately.
22. In case on fire or hearing emergency siren, evacuate the laboratory
quickly in orderly manner. In case of small accident or small fire occurs, try
to overcome them wisely.
23. Report any accidents (minor or major) to your laboratory instructor. This
accident must be recorded in log book “CATATAN KEMALANGAN”.
24. Clean all glassware and place them appropriately before leaving laboratory.
Make sure to switch off instruments, water and gas supplies.
25. Final year project students should require permission from supervisor and
head of department if they want to work after normal working hours.
AN EXPERIMENT DONE WELL IS......
AN EXPERIMENT DONE SAFELY
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
. 4
, [Type text]
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
GUIDELINE TO WRITE LAB REPORT
OBJECTIVE
State the objective clearly in a complete sentence. A few explanatory sentences
may be included, if needed.
The objective should answer the question: What is the lab objective designed to determine?
EXPERIMENTAL PROCEDURE
The experimental methods should give a detailed description in your own words of
how YOU accomplished the experimentation (Passive voice sentences).
*Tips: This should include equipment used in the experiment as well as how it was
used. The description should have sufficient detail so that another experimenter
could duplicate your efforts.
Use sketches and diagrams to describe the experimental set-up. Label the main
components.
Tips: Any information copied directly from Lab manuals or other references should be stated
within quotes and referred, otherwise, it is considered plagiarism.
RESULTS AND DISCUSSION
This is the most important part of the report. Summarize your results in the
introductory sentence. Relate your results to your objective. Present the results in
the easiest way for your reader to understand: graphs, tables, figures, etc.
Spreadsheets are often an ideal tool for organizing the data, analyzing the data, and
generating graphs and tables. All tables and figures should be accompanied by
comments or discussions in the text of report; use a numbering system for
identification of each one.
All figures and tables must have numbers and captions. While the table captions
should be placed over the table, figure captions should be placed below the figure.
SKA3013 LABORATORY MANUAL
ANALYTICAL CHEMISTRY 2022/2023
new update
. 5
