BIOCHEM C785 2 OA READINESS CHECK CORRECT QUESTIONS AND ANSWERS

BIOCHEM C785 2 OA READINESS CHECK
CORRECT QUESTIONS AND ANSWERS

What is the complementary sequence to 3’ ATG CGA ATA 5’?(0/1 Points)

3’ TAT TCG CAT 5’
3’ TAC GCT TAT 5’


The correct answer is 3' TAT TCG CAT 5'. Remember complementary means
“Matching or Pairing” You have to remember to pay attention to your numbers as well
as your letters (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original
sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the
3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’ If you
chose 3' TAC GCT TAT 5', this is incorrect. Although the nucleotides are in the correct
order, the 5' and 3' are in the wrong place. DNA strands that are complementary run in
opposite directions. This means that if one strand starts with a 3', the the
complementary strand must start with a 5'. If none of the answer choices give the
correct answer in 5' ---> 3', then you need towrite its mirror, which will run from 3' -
--> 5'.

3’ GAT AGC ATA 5’
5’ ATA AGC GTA 3’


2
Which amino acid sequence would result from this Coding DNA strand?3’ ATG
CAG ATA 5’?
(1/1 Points)
Tyr Val
TyrIle
Gln Ile
Ile Asp
Val
The correct answer is Ile Asp Val. We are starting at the coding strand, and have
to
remember the relationship between coding DNA and mRNA. These two strands are

, BIOCHEM C785 2 OA READINESS CHECK
CORRECT QUESTIONS AND ANSWERS

non- complementary and parallel. So we copy the coding strand , change T ---> U, and
then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’
mRNAMirror bychanging orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val
(chart is in direction of 5' ---> 3')

Leu His Lys

, BIOCHEM C785 2 OA READINESS CHECK
CORRECT QUESTIONS AND ANSWERS


3
Which amino acid sequence would be made from this template DNA strand? 5’
TAT TAC CGA 3’?
(1/1 Points)
Tyr Tyr
ArgIle
Met Ala
Ser Val Ile
The correct answer is Ser Val Ile because 5’ TAT TAC CGA 3’ template is
complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong
orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile

Ser His Gln
4
Which of the following are the correct components for a PCR reaction? (1/1 Points)

dNTPs, Primer, RNA Polymerase, template RNA

, BIOCHEM C785 2 OA READINESS CHECK
CORRECT QUESTIONS AND ANSWERS

dNTPs, Primer, DNA Polymerase, template DNA
The correct answer is dNTPs, Primer, DNA Polymerase, template DNA. Notice that all
components are about DNA. The Primer is even a DNA Primer.

ATP, Primer, mRNA polymerase; template mRNA

Acetyl CoA, RNA primer, DNA Ligase, Template phosphate
5
Assuming 100% reaction efficiency, how many DNA copies will be produced after 5 PCR



16

32
The correct answer is 32.

2x2x2x2x2=3264

10
6
Which of the following would represent a silent mutation if this is the original sequence:
(1/1 Points)

5’ AUC GGA ACA 3’

5' AUA GUA ACA 3'
The correct answer is 5' AUC GUA ACA 3' Ile
Val Thr 5’ AUG GUA ACA 3’
5’ AUC GCA ACA 3’
7
If the original coding sequence is 5’ CGA TAC TTC AGA 3’ and it is mutated to 5' CGA TAT
(1/1 Points)



Silent
The correct answer is silent mutation. The nucleotide sequence changes, but it codes
forthe same amino acid. The coding sequence 5' TAC 3' corresponds to the mRNA
sequence 5' UAC 3' (Tyr), and the coding sequence 5' TAT 3' corresponds to the
8
mRNA sequence 5' UAU 3' (Tyr). Since the C changed to at T, this is a point
Huntington's is an autosomal dominant disorder. Which of the following