TCA no no no no no no Glucose
Citrate synthase is the f rst enzyme n the TCA cycle that
3
comb nes Acetyl Co-A w th OAA to form c trate. And it's
h ghly regulated. Hav ng five alloster c sites, four nh bitor
sites, and one activator site.
YIP Glycolysis
2 pyruvate
The second enzyme is Acton tase, wh ch isomerases the
c trate to isoc trate. Reversible react on.
The th rd enzyme is isoc trate Dehydrogenase. Every time
you hear of D.H., it means convert ng NAD+ or FAD to NADH
or FADH2. Th s enzyme also extracts one carbon form CO2.
Irreversible react on. Th s enzyme has three alloster c sites:
two stimulat on sites and one nh bitor. Calc um is a
stimulator for th s enzyme because it helps muscles to
generate more ATP and contract.
2NADH 2 acetyl Co A
Ftrat
The fourth enzyme is a-KG D.H. it w ll convert a-KG to succ nyl
Co-A. By D.H. NAD+ and pop off one carbon to form CO2. It's PDA complex
nh bited by the h gh concentrat on of products Succ nyl Co-A
BI
iris
and NADH. And stimulate by Ca+2. Recall that the PDH
complex enzyme when b nds Co-A to pyruvate. It's the exact Oxaloacetate com
mechan sm w th a-KG D.H. enzyme. It b nds Co-A to a-KG and
gets r d of one carbon molecule. Irreversible react on.
Then, n the follow ng react on, we get r d of Co-A and form
ATP by substrate phosphorylat on. By fus ng Pi + GDP then,
i
I D Al
ADP takes the Phosphate from GTP. The enzyme nvolved n
th s react on is called Succ nyl CoA synthetase, convert ng
Succ nyl CoA to Succ nate. Reversible react on. NADH
The succ nate D.H. Enzyme converts succ nate to fumarate
T NAD
by dehydrogenase FAD to FADH2. It's b nd ng to the nner
Citrate synthase is the f rst enzyme n the TCA cycle that
3
comb nes Acetyl Co-A w th OAA to form c trate. And it's
h ghly regulated. Hav ng five alloster c sites, four nh bitor
sites, and one activator site.
YIP Glycolysis
2 pyruvate
The second enzyme is Acton tase, wh ch isomerases the
c trate to isoc trate. Reversible react on.
The th rd enzyme is isoc trate Dehydrogenase. Every time
you hear of D.H., it means convert ng NAD+ or FAD to NADH
or FADH2. Th s enzyme also extracts one carbon form CO2.
Irreversible react on. Th s enzyme has three alloster c sites:
two stimulat on sites and one nh bitor. Calc um is a
stimulator for th s enzyme because it helps muscles to
generate more ATP and contract.
2NADH 2 acetyl Co A
Ftrat
The fourth enzyme is a-KG D.H. it w ll convert a-KG to succ nyl
Co-A. By D.H. NAD+ and pop off one carbon to form CO2. It's PDA complex
nh bited by the h gh concentrat on of products Succ nyl Co-A
BI
iris
and NADH. And stimulate by Ca+2. Recall that the PDH
complex enzyme when b nds Co-A to pyruvate. It's the exact Oxaloacetate com
mechan sm w th a-KG D.H. enzyme. It b nds Co-A to a-KG and
gets r d of one carbon molecule. Irreversible react on.
Then, n the follow ng react on, we get r d of Co-A and form
ATP by substrate phosphorylat on. By fus ng Pi + GDP then,
i
I D Al
ADP takes the Phosphate from GTP. The enzyme nvolved n
th s react on is called Succ nyl CoA synthetase, convert ng
Succ nyl CoA to Succ nate. Reversible react on. NADH
The succ nate D.H. Enzyme converts succ nate to fumarate
T NAD
by dehydrogenase FAD to FADH2. It's b nd ng to the nner
