BIOCHEM C785 Final Exam

Which mRNA sequence results in a polypeptide that contains three amino acids? 5' - CUU GAU ACU UAG - 3' 5' - UAU GCC CUA CGA - 3' 5' - AUC CAG UAA AAG - 3' 5' - GUC UGA CAC CGG - 3' Correct Answer: 5' - CUU GAU ACU UAG - 3' Which RNA sequence would end in the following polypeptide? Phe-Thr-Val-Stop 5' - UUC ACA GUA UAA - 3' 5' - UUU UAU GUU UGG - 3' 5' - UUC CAU UGA GUA - 3' 5' - AAU AUG ACA CUU - 3' Correct Answer: 5' - UUC ACA GUA UAA - 3' What is the complementary sequence for the following DNA sequence? 5' - GCC ATC TCG AAT - 3' 5' - CGG TAG AGC TTA - 3' 5' - ATT CGA GAT GGC - 3' 5' - TAA CGA GAT CCG - 3' 5' - CGG ATG TCG TTA - 3' Correct Answer: 5' - ATT CGA GAT GGC - 3' Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four complete PCR cycles? 24 16 8 4 Correct Answer: 16 What is the function of DNA polymerase in the process of PCR? It separates the two DNA strands so that replication may occur. It recognizes the primers and uses the available dNTPs to replicate the template DNA sequence. It synthesizes primers and uses the available dNTPs to replicate the template DNA sequence. It links the products of PCR together to generate a complete strand. Correct Answer: It recognizes the primers and uses the available dNTPs to replicate the template DNA sequence. 5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA 5' - [...] ATT CGC GAG GGC [...] - 3' −