C785 Biochemistry preassessment

Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four complete PCR cycles? Correct Answer: 16 What is the function of DNA polymerase in the process of PCR? Correct Answer: It recognizes the primers and uses the available dNTPs to replicate the template DNA sequence. What are possible blood types of the parents of a patient with blood type AB? Correct Answer: A and B − Amino Acid Number Tyr Gly Thr Pro − Amino Acid Sequence TAC GGG ACA CCA − DNA Sequence What is the amino acid in position 118 that results from a single nucleotide insertion immediately following the three guanines that encode for glycine 116? Correct Answer: Thr In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to valine (Val). What change in codons corresponds to this mutation? Correct Answer: GAA to GTA Which type of mammalian DNA damage repair requires the presence of the chromosome that is homologous to the damaged chromosome? Correct Answer: Recombination Xeroderma pigmentosum (XP) is a recessive genetic disease that occurs when one or more of the genes that perform nucleotide excision repair are nonfunctional. Why do XP patients have a much higher incidence of skin cancer than the general population? Correct Answer: The mutation rate of all other genes is higher due to failure to repair. Which mRNA sequence results in a polypeptide that contains three amino acids? Correct Answer: 5' - CUU GAU ACU UAG - 3' Which template strand DNA sequence would result in the amino acid (Ser)? Correct Answer: 5' - CGA - 3' What is the complementary sequence for the following DNA sequence? 5' - GCC ATC TCG AAT - 3' Correct Answer: 5' - ATT CGA GAT GGC - 3' Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is associated with chronic exposure to cocaine and contributes to drug addicti