Academische Commu-
nicatie B-B2AS17
Result and discussion section
CRISPR-cas9 efficiently cleaves HBV DNA in the
treatment for hepatitis B virus
In this study the application of the CRISPR-cas9 system on the targeting and cleavage of Hepadnaviridae cc-
cDNA was examined.
Maartje Koppies
1
Universiteit Utrecht, bachelor biologie
Results (classic)
gRNA 3 and 4 most efficient in decreasing antigen concentration
In the first experiment the efficiency of four different guide RNAs, that were able to
target the HBV DNA sequences at different places, was evaluated (1,2,3,4). Firstly, in
the control group an HBV surface antigen (HBsAg) concentration of 8.91 ng/ml +/-
0.2 (n = 20) was measured in the medium of the HBV infected cell lines. Secondly,
the concentration HBsAg that was measured with gRNA 1 (9,24 +/- 0.005 ng/ml; n =
20, p = 0.33) was higher than the control group C. The third gRNA showed a concen-
Citation: Lastname, F.; tration of 3.3 +/- 0.1 ng/ml HBsAg (n=20, p = 0.002)
Lastname, F.; Lastname, F.
Title. Biology 2022, 11, x. And finally, gRNA 4 led to the biggest decrease of antigen con-
https://doi.org/10.3390/xxxxx
centration (2.64 +/- 0.2 ng/ml HbsAg; n = 20, p = 0.002) (Fig-
Academic Editor: Firstname
Lastname
ure 1).
Received: date
Accepted: date
Published: date
Publisher’s Note: MDPI stays
neutral with regard to
jurisdictional claims in
published maps and
institutional affiliations.
Copyright: © 2022 by the authors. Sub-
mitted for possible open access publica-
tion under the terms and conditions of
the Creative Commons Attribution (CC
BY) license (https://creativecommon-
s.org/licenses/by/4.0/).
Academic Communication 2022, 11, x. https://doi.org/10.3390/xxxxx
nicatie B-B2AS17
Result and discussion section
CRISPR-cas9 efficiently cleaves HBV DNA in the
treatment for hepatitis B virus
In this study the application of the CRISPR-cas9 system on the targeting and cleavage of Hepadnaviridae cc-
cDNA was examined.
Maartje Koppies
1
Universiteit Utrecht, bachelor biologie
Results (classic)
gRNA 3 and 4 most efficient in decreasing antigen concentration
In the first experiment the efficiency of four different guide RNAs, that were able to
target the HBV DNA sequences at different places, was evaluated (1,2,3,4). Firstly, in
the control group an HBV surface antigen (HBsAg) concentration of 8.91 ng/ml +/-
0.2 (n = 20) was measured in the medium of the HBV infected cell lines. Secondly,
the concentration HBsAg that was measured with gRNA 1 (9,24 +/- 0.005 ng/ml; n =
20, p = 0.33) was higher than the control group C. The third gRNA showed a concen-
Citation: Lastname, F.; tration of 3.3 +/- 0.1 ng/ml HBsAg (n=20, p = 0.002)
Lastname, F.; Lastname, F.
Title. Biology 2022, 11, x. And finally, gRNA 4 led to the biggest decrease of antigen con-
https://doi.org/10.3390/xxxxx
centration (2.64 +/- 0.2 ng/ml HbsAg; n = 20, p = 0.002) (Fig-
Academic Editor: Firstname
Lastname
ure 1).
Received: date
Accepted: date
Published: date
Publisher’s Note: MDPI stays
neutral with regard to
jurisdictional claims in
published maps and
institutional affiliations.
Copyright: © 2022 by the authors. Sub-
mitted for possible open access publica-
tion under the terms and conditions of
the Creative Commons Attribution (CC
BY) license (https://creativecommon-
s.org/licenses/by/4.0/).
Academic Communication 2022, 11, x. https://doi.org/10.3390/xxxxx
