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Quiz 2 Biochemistry Lab Chem 378|100% correct
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Quiz 2 Biochemistry Lab Chem 378 Name:____________________ 1. Below are restriction enzyme maps of two plasmids. Draw or described in numbers the expected bands of digestion in 1% agarose gel electrophoresis, relative to the 2 bands identified for you as control. 2. Explain the different steps that are involved in a PCR experiment (include all the reagents used in the PCR mixture). 5.5 kb BamHI 3730 PstI 2650 BamHI 3050 PstI 1720 PstI BamH1 PstI BamHI pCAG-EYFP 4000 bp 3000 bp 2000 bp 800 bp 400 bp 200 bp pBS(+)325 BamHI Xmn1 BamHI + Xmn1 (3005) (905) This study source was downloaded by from CourseH on :34:15 GMT -06:00 2. Explain the different steps that are involved
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quiz 2 biochemistry lab chem 378
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