BioE 10 Homework 11|100% correct

BioE 10 Homework 11 1. (_____ out of 2 points) The following picture is from Google Maps showing a plane in flight over Hyde Park. It was taken with a CCD camera that takes four images - one each for the blue, green, and red color channels, followed by another for luminosity, with a delay between each. The four images are then combined into one. The plane is a Boeing 747, and is 75 meters from tip to tail. You’ve narrowed down the type of camera that took the images to two types. Type A, which has a delay of 0.35 s between the different channels, and Type B, which has a delay of 0.06 s between the different channels. Which camera, Type A or Type B, was used to take this picture, and why? (Include an informal reference to a journal, web page, etc. if necessary.) Type B – A Boeing 747, according to the Microsoft Encarta Encyclopedia from 2000, has a cruising speed of 885 km/hr, which is standardized as 245 m/s. At this speed, the plane would travel roughly 86 meters in 0.35 seconds and roughly 15 meters in 0.06 seconds. Given the distance between each of the images of the plane following the delay, it is clear that the delay cannot be as large as 0.35 seconds, and it has to be the much smaller 0.06 second delay of camera Type B. 2. (_____ out of 3 points) Download and install the ImageJ image analysis software. On bcourses you will find an uncompressed TIF image file of pellets of bacteria in wells of a 96-well plate (“RFP assay - BioE ”). These wells have been photographed while exposed to UV light, using an appropriate filter, so the brightness of each pellet in the TIF corresponds to the quantity of “red” (e.g., photons of certain wavelengths) being emitted from the pellet and captured by the camera. The bacteria in each separate well have been transformed with a This study source was downloaded by from CourseH on :26:49 GMT -06:00 different plasmid designed to produce red fluorescent protein (RFP) - some plasmids produce more RFP, some produce less. You’ve been tasked to quantify the fluorescence of each pellet using the following protocol. Note that the image is in black and white because it contains only the red channel from a color image. ● Open the image in ImageJ. ● Go to Analyze Set Measurements and make sure that "Integrated Density" is checked. Integrated density simply adds up the brig