Introduction to
Cloning
, Background
• In 1960s work on Bacteriophage lambda noted
‘recombination’ of phage DNA into host
chromosome
• Arber et al noted the restriction modification
system
• 1970 Hamilton Smith purifies restriction enzyme
from Haemophilus influenza
• Daniel Nathans uses restriction enzymes to map
the simian virus 40 genome
• 1972 Paul Berg created the first
recombinant DNA molecules
• 1973 Herbert Boyer and Stanley
Cohen create the first recombinant
organisms
, Molecular Cloning of DNA
1. Isolation of DNA fragments
2. Selecting a DNA cloning vector
3. Joining fragments of DNA together
4. Transferring recombinant DNA into host cell
5. Identifying cells which contain recombinant
DNA
Cloning
, Background
• In 1960s work on Bacteriophage lambda noted
‘recombination’ of phage DNA into host
chromosome
• Arber et al noted the restriction modification
system
• 1970 Hamilton Smith purifies restriction enzyme
from Haemophilus influenza
• Daniel Nathans uses restriction enzymes to map
the simian virus 40 genome
• 1972 Paul Berg created the first
recombinant DNA molecules
• 1973 Herbert Boyer and Stanley
Cohen create the first recombinant
organisms
, Molecular Cloning of DNA
1. Isolation of DNA fragments
2. Selecting a DNA cloning vector
3. Joining fragments of DNA together
4. Transferring recombinant DNA into host cell
5. Identifying cells which contain recombinant
DNA
