Name: Hiba Majid 13E (P:\Science\Mr Alim\Year 12\AS Biology)
QUESTION TO BE INVESTIGATED: Effect of temperature on initial rate of enzyme activity
METHOD
1. Carry out instructions given (or that you have produced/researched).
List apparatus and describe techniques used when measuring quantities: (CPAC1)
Piece of apparatus Purpose of piece of Comment on how piece of equipment Appendix
apparatus was used 5c
accurately/appropriately/safely technique
Potato Contains the enzyme, Since it may contain bacteria, hands
catalase, which we will be should be washed properly before and
investigating (it breaks after use, use sanitiser after.
down hydrogen peroxide)
Mass will be weighed accurately using
a weighing scale
Mortar and pestle Used to mash potatoes Since they are ceramic, they should be
with distilled water to kept away from the edge of the table
release catalase enzymes to avoid content spilling or breaking
Hydrogen peroxide This is the substrate that May cause harm/irritation to eyes or
the catalase will be in cuts. Wear goggles, lab coats and
breaking down into goggles, and avoid contact with skin
oxygen (and water)
Stop watch This will be used to time One person will be managing the stop
the reaction in minutes. watch only to ensure no mistakes are
At each one minute made and it is stopped on time and
interval, the volume of when we are meant to record a
gas will be measured volume. Time is measured in seconds
Water bath this will be used to This will be kept in the middle of the
change the temperature table to stop it falling over
environment of the
enzymes to see how
temperature affects their
activity
Boiling tube This will hold the
potatoes and hydrogen
peroxide inside the water
bath
Mr Alim
, Name: Hiba Majid 13E (P:\Science\Mr Alim\Year 12\AS Biology)
Bung and delivery This will be placed on top We will ensure the bung is on securely
tube of the tube with the to avoid oxygen leaking out
potatoes and hydrogen
peroxide to collect the
oxygen and stop it from
leaking out
Beaker This will hold the This will be kept in the middle of the
measuring cylinder which table to avoid it falling over
is measuring the volume
of oxygen
Measuring cylinder This will be filled with This will be held down by a weight in
water. As the oxygen the large beaker to avoid it tilting
collects it puts pressure
on the water causing it to
go out of the measuring
cylinder, allowing the
volume of oxygen to be
calculated.
Gas syringe To gather the oxygen gas A danger might result when too much
that is generated when gas has developed and popped off the
catalase and H2O2 react syringe. Utilise a retort stand and
clamps to secure. (Medium risk)
Cork borer To remove the potato's Use a white tile to separate the potato
cylindrical core cores and discard them to prevent
infection since the pressure needed to
insert the cork into the potato might
be harmful owing to its sharp edges.
(Medium risk)
Scalpel To cut potatoes into To cut the potatoes into desired size. It
smaller pieces will be kept away from the edge of the
table to stop it from falling and
potentially stabbing someone. After
use, it will be placed back into its
appropriate place, away from
students/teachers.
Thermometer To measure water bath Will be handled carefully to avoid it
temperature from breaking and hurting someone
with the glass
Mr Alim
QUESTION TO BE INVESTIGATED: Effect of temperature on initial rate of enzyme activity
METHOD
1. Carry out instructions given (or that you have produced/researched).
List apparatus and describe techniques used when measuring quantities: (CPAC1)
Piece of apparatus Purpose of piece of Comment on how piece of equipment Appendix
apparatus was used 5c
accurately/appropriately/safely technique
Potato Contains the enzyme, Since it may contain bacteria, hands
catalase, which we will be should be washed properly before and
investigating (it breaks after use, use sanitiser after.
down hydrogen peroxide)
Mass will be weighed accurately using
a weighing scale
Mortar and pestle Used to mash potatoes Since they are ceramic, they should be
with distilled water to kept away from the edge of the table
release catalase enzymes to avoid content spilling or breaking
Hydrogen peroxide This is the substrate that May cause harm/irritation to eyes or
the catalase will be in cuts. Wear goggles, lab coats and
breaking down into goggles, and avoid contact with skin
oxygen (and water)
Stop watch This will be used to time One person will be managing the stop
the reaction in minutes. watch only to ensure no mistakes are
At each one minute made and it is stopped on time and
interval, the volume of when we are meant to record a
gas will be measured volume. Time is measured in seconds
Water bath this will be used to This will be kept in the middle of the
change the temperature table to stop it falling over
environment of the
enzymes to see how
temperature affects their
activity
Boiling tube This will hold the
potatoes and hydrogen
peroxide inside the water
bath
Mr Alim
, Name: Hiba Majid 13E (P:\Science\Mr Alim\Year 12\AS Biology)
Bung and delivery This will be placed on top We will ensure the bung is on securely
tube of the tube with the to avoid oxygen leaking out
potatoes and hydrogen
peroxide to collect the
oxygen and stop it from
leaking out
Beaker This will hold the This will be kept in the middle of the
measuring cylinder which table to avoid it falling over
is measuring the volume
of oxygen
Measuring cylinder This will be filled with This will be held down by a weight in
water. As the oxygen the large beaker to avoid it tilting
collects it puts pressure
on the water causing it to
go out of the measuring
cylinder, allowing the
volume of oxygen to be
calculated.
Gas syringe To gather the oxygen gas A danger might result when too much
that is generated when gas has developed and popped off the
catalase and H2O2 react syringe. Utilise a retort stand and
clamps to secure. (Medium risk)
Cork borer To remove the potato's Use a white tile to separate the potato
cylindrical core cores and discard them to prevent
infection since the pressure needed to
insert the cork into the potato might
be harmful owing to its sharp edges.
(Medium risk)
Scalpel To cut potatoes into To cut the potatoes into desired size. It
smaller pieces will be kept away from the edge of the
table to stop it from falling and
potentially stabbing someone. After
use, it will be placed back into its
appropriate place, away from
students/teachers.
Thermometer To measure water bath Will be handled carefully to avoid it
temperature from breaking and hurting someone
with the glass
Mr Alim
