The effect of H2O2, FeSO4 and ascorbic acid on
lipid peroxidation in pig liver homogenate.
Faculty of Health, Medicine and Life Sciences (FHML)
Maastricht University
Course BBS2002
Practical training session: 9th November, 2022
11th January, 2023
, Introduction
Life expectancy in the United States has increased dramatically over the past 200
years, reaching nearly 80 years, due to vast improvements and understanding in
healthcare, sanitation, drinkable water, nutrition, and quality of life (1). Aging is an
unavoidable process that is defined by the gradual loss of physiological integrity,
which impairs function and increases mortality risk. The hallmarks of aging
summarize the processes that occur and accumulate when getting older (2-3).
One of the hallmarks of aging is mitochondrial dysfunction, which is driven by
reactive oxygen species (ROS) leading to oxidative stress and damaging DNA,
proteins, lipids, organs, and tissues (4). ROS is created by the partial reduction of
molecular oxygen (O2) to superoxide (O2-), hydrogen peroxide (H2O2), lipid peroxides
(ROOH) or the equivalent hydroxyl (HO.) (5-6). Oxidative stress contributes,
according to studies, to a number of diseases, including atherosclerosis, cancer,
chronic obstructive pulmonary disease (COPD), Alzheimer’s disease and
cardiovascular disease (7).
Lipid peroxidation refers to the process whereby ROS and lipids react.
Polyunsaturated fatty acids are primarily oxidized by lipid peroxidation. Lipid
peroxidation takes place in cells through Fenton-like reactions. Many different
Fenton-like reactions have now been found that alter the peroxide, metal ion and
water ligand. The classic Fenton reaction is the reaction between Fe 2+ or Fe3+ and
H2O2, to create one free radical. Nevertheless, iron ions are mostly used in cells to
carry out these Fenton-like reactions (8).
Fig 1 The mechanism of classical Fenton reaction (9).
lipid peroxidation in pig liver homogenate.
Faculty of Health, Medicine and Life Sciences (FHML)
Maastricht University
Course BBS2002
Practical training session: 9th November, 2022
11th January, 2023
, Introduction
Life expectancy in the United States has increased dramatically over the past 200
years, reaching nearly 80 years, due to vast improvements and understanding in
healthcare, sanitation, drinkable water, nutrition, and quality of life (1). Aging is an
unavoidable process that is defined by the gradual loss of physiological integrity,
which impairs function and increases mortality risk. The hallmarks of aging
summarize the processes that occur and accumulate when getting older (2-3).
One of the hallmarks of aging is mitochondrial dysfunction, which is driven by
reactive oxygen species (ROS) leading to oxidative stress and damaging DNA,
proteins, lipids, organs, and tissues (4). ROS is created by the partial reduction of
molecular oxygen (O2) to superoxide (O2-), hydrogen peroxide (H2O2), lipid peroxides
(ROOH) or the equivalent hydroxyl (HO.) (5-6). Oxidative stress contributes,
according to studies, to a number of diseases, including atherosclerosis, cancer,
chronic obstructive pulmonary disease (COPD), Alzheimer’s disease and
cardiovascular disease (7).
Lipid peroxidation refers to the process whereby ROS and lipids react.
Polyunsaturated fatty acids are primarily oxidized by lipid peroxidation. Lipid
peroxidation takes place in cells through Fenton-like reactions. Many different
Fenton-like reactions have now been found that alter the peroxide, metal ion and
water ligand. The classic Fenton reaction is the reaction between Fe 2+ or Fe3+ and
H2O2, to create one free radical. Nevertheless, iron ions are mostly used in cells to
carry out these Fenton-like reactions (8).
Fig 1 The mechanism of classical Fenton reaction (9).
