The University of Manitoba
Practice Test
Final Examination
MBIO1010: Microbiology I
Time: 2 hours
READ THE FOLLOWING INSTRUCTIONS CAREFULLY:
• This test consists of 75 multiple choice questions that must be answered on your
bubble sheet.
• You must return your test paper AND your bubble sheet when you are finished. •
Your NAME and STUDENT NUMBER must appear on both the test paper and
the bubble sheet.
Name Student number
1. Which of the following is a reason in support of the importance of
microorganisms:
a. Microorganisms are limited to very moderate conditions on Earth b.
Microbes are only able to participate in very select biogeochemical cycles c.
Microorganisms are the most abundant lifeform on Earth
d. Microorganisms are dependent on plants for the production of useable
nitrogen
e. More than one of the above are reasons in support of the importance of
microorganisms
2. Which of the following is correctly matched?
a. Prokaryotes: are mostly multi-cellular
b. Eukaryotes: divide by meiosis, mitosis and binary fission
c. Eukaryotes: include archaea, bacteria and protozoa
d. Prokaryotes: lack a membrane-bound nucleus but contain membrane
bound organelles such as mitochondria
e. None of the above are correctly matched
3. Simple staining:
a. Uses two dyes to enhance an image
b. Can make use of negatively charged basic dyes
c. Can make use of positively charged basic dyes
d. Can make use of positive stains to color the background
e. None of the above are true of simple stains
4. PHB granules:
a. Store glucose inside of the bacterial cell
b. Store sulfur inside of the bacterial cell
c. Store phosphate inside of the bacterial cell
d. Store carbon inside of the bacterial cell
e. Store gas inside of the bacterial cell
, Page 1 of 17
The University of Manitoba
Practice Test
Final Examination
MBIO1010: Microbiology I
Time: 2 hours
5. Which of the following statements is incorrect of Pasteur’s famous swan neck
flask experiment?
a. He was able to demonstrate that spontaneous generation is not possible
b. He placed a cap on the flask to prevent new bacteria from entering the
nutrient broth
c. The broth was heated to destroy any contaminating bacteria that were
present in the broth at the start of the experiment
d. He created the swan neck in order to prevent new bacteria from entering
the nutrient broth
e. None of the above. Options a-d are all correct of Pasteur’s swan neck flask
experiment
6. Which of the following cannot be found in a gram negative cell?
a. Peptidoglycan
b. Outer membrane
c. Endotoxin
d. Lipoteichoic acid
e. Plasma membrane
7. Evidence to support the theory that chloroplasts have evolved from
prokaryotic cells include:
a. 80S ribosomes inside of chloroplast structures
b. The presence of a circular chromosome rich in histone proteins
c. The presence of linear chromosomes lacking in histone proteins
d. Porins located in the outer membrane of chloroplasts
e. All of the above support the theory that chloroplasts evolved from
prokaryotic cells
8. MOT proteins:
a. Move protons from the cytoplasm of the cell to the outside, releasing
energy used to rotate the flagellar filament
b. Form the filament of the flagellum
c. Are used to attach the filament to the basal body of the flagellum d.
Can be removed from the cell and the flagellum will still demonstrate
rotation
, e. None of the above are true of MOT proteins
9. 70S ribosomes:
a. Have a small 40S subunit
b. Are found inside of chloroplasts
c. Have a large 60S subunit
d. Can be found in association with the plasma membrane when they are
responsible for assembling proteins destined to be excreted from the cell e.
None of the above are true of prokaryotic ribosomes
Page 2 of 17
The University of Manitoba
Practice Test
Final Examination
MBIO1010: Microbiology I
Time: 2 hours
10. Prokaryotic cells:
a. Grow more slowly than larger cells
b. Do not support the efficient uptake of nutrients
c. Are typically very large when they are pathogenic
d. Have a very small surface area to volume ratio
e. None of the above are true of prokaryotic cells
11. Consider the image shown. The
organism growing under the label B
would best be described as:
a. Alpha hemolytic
b. Beta hemolytic
c. Gamma hemolytic
d. Non-hemolytic
e. Not enough information is provided to answer this question
12. Phosphorous is an important component of all of the following
except: a. Glucose
b. DNA
c. RNA
d. Phospholipids
e. More than one of the above do not contain phosphorous
13. Boiling water:
a. Would achieve true sterilization of a sample that was contaminated with
Clostridium botulinum
b. Destroys microorganisms by denaturing proteins and other
macromolecules
c. Provides more intense conditions than the autoclave
d. Is a bacteriostatic form of antimicrobial activity
Practice Test
Final Examination
MBIO1010: Microbiology I
Time: 2 hours
READ THE FOLLOWING INSTRUCTIONS CAREFULLY:
• This test consists of 75 multiple choice questions that must be answered on your
bubble sheet.
• You must return your test paper AND your bubble sheet when you are finished. •
Your NAME and STUDENT NUMBER must appear on both the test paper and
the bubble sheet.
Name Student number
1. Which of the following is a reason in support of the importance of
microorganisms:
a. Microorganisms are limited to very moderate conditions on Earth b.
Microbes are only able to participate in very select biogeochemical cycles c.
Microorganisms are the most abundant lifeform on Earth
d. Microorganisms are dependent on plants for the production of useable
nitrogen
e. More than one of the above are reasons in support of the importance of
microorganisms
2. Which of the following is correctly matched?
a. Prokaryotes: are mostly multi-cellular
b. Eukaryotes: divide by meiosis, mitosis and binary fission
c. Eukaryotes: include archaea, bacteria and protozoa
d. Prokaryotes: lack a membrane-bound nucleus but contain membrane
bound organelles such as mitochondria
e. None of the above are correctly matched
3. Simple staining:
a. Uses two dyes to enhance an image
b. Can make use of negatively charged basic dyes
c. Can make use of positively charged basic dyes
d. Can make use of positive stains to color the background
e. None of the above are true of simple stains
4. PHB granules:
a. Store glucose inside of the bacterial cell
b. Store sulfur inside of the bacterial cell
c. Store phosphate inside of the bacterial cell
d. Store carbon inside of the bacterial cell
e. Store gas inside of the bacterial cell
, Page 1 of 17
The University of Manitoba
Practice Test
Final Examination
MBIO1010: Microbiology I
Time: 2 hours
5. Which of the following statements is incorrect of Pasteur’s famous swan neck
flask experiment?
a. He was able to demonstrate that spontaneous generation is not possible
b. He placed a cap on the flask to prevent new bacteria from entering the
nutrient broth
c. The broth was heated to destroy any contaminating bacteria that were
present in the broth at the start of the experiment
d. He created the swan neck in order to prevent new bacteria from entering
the nutrient broth
e. None of the above. Options a-d are all correct of Pasteur’s swan neck flask
experiment
6. Which of the following cannot be found in a gram negative cell?
a. Peptidoglycan
b. Outer membrane
c. Endotoxin
d. Lipoteichoic acid
e. Plasma membrane
7. Evidence to support the theory that chloroplasts have evolved from
prokaryotic cells include:
a. 80S ribosomes inside of chloroplast structures
b. The presence of a circular chromosome rich in histone proteins
c. The presence of linear chromosomes lacking in histone proteins
d. Porins located in the outer membrane of chloroplasts
e. All of the above support the theory that chloroplasts evolved from
prokaryotic cells
8. MOT proteins:
a. Move protons from the cytoplasm of the cell to the outside, releasing
energy used to rotate the flagellar filament
b. Form the filament of the flagellum
c. Are used to attach the filament to the basal body of the flagellum d.
Can be removed from the cell and the flagellum will still demonstrate
rotation
, e. None of the above are true of MOT proteins
9. 70S ribosomes:
a. Have a small 40S subunit
b. Are found inside of chloroplasts
c. Have a large 60S subunit
d. Can be found in association with the plasma membrane when they are
responsible for assembling proteins destined to be excreted from the cell e.
None of the above are true of prokaryotic ribosomes
Page 2 of 17
The University of Manitoba
Practice Test
Final Examination
MBIO1010: Microbiology I
Time: 2 hours
10. Prokaryotic cells:
a. Grow more slowly than larger cells
b. Do not support the efficient uptake of nutrients
c. Are typically very large when they are pathogenic
d. Have a very small surface area to volume ratio
e. None of the above are true of prokaryotic cells
11. Consider the image shown. The
organism growing under the label B
would best be described as:
a. Alpha hemolytic
b. Beta hemolytic
c. Gamma hemolytic
d. Non-hemolytic
e. Not enough information is provided to answer this question
12. Phosphorous is an important component of all of the following
except: a. Glucose
b. DNA
c. RNA
d. Phospholipids
e. More than one of the above do not contain phosphorous
13. Boiling water:
a. Would achieve true sterilization of a sample that was contaminated with
Clostridium botulinum
b. Destroys microorganisms by denaturing proteins and other
macromolecules
c. Provides more intense conditions than the autoclave
d. Is a bacteriostatic form of antimicrobial activity
