BIOL 1409 lab safety and microscope review study guid
exam 2022
Objectives:
Develop good laboratory practices.
Properly use Personal Protective Equipment (PPE).
Identify the location and proper use of emergency equipment.
Identify when a stereomicroscope (dissecting microscope) versus
a compound light microscope would be used in the lab
Describe the major differences between light microscopes and electron
microscopes
Identify the parts of a compound light microscope and stereomicroscope
Describe the proper way to carry a microscope
Determine the total magnification of an object being viewed by
a compound light microscope if given the ocular lens and
objective lens magnifications
Executing the techniques to prepare wet mounts of animal and plant
tissues
I. Introduction
The laboratory is a fun place in which we can explore many concepts that we
are learning in the lecture portion of the course. We want to make sure that
every single one of you can explore the different topics in a safe manner. In
this lab you will learn about some lab safety rules that you must follow. Many
of the labs will require to use of microscopes. In this lab you will also learn
correct use and care of the laboratory equipment. All students working in the
laboratory are responsible for maintaining equipment and materials in proper
working conditions. Let us explore!
II. Lab Safety
Laboratory safety involves all the measures taken by the laboratory worker,
laboratory owner, institution, and regulatory agencies to eliminate potential
harm to human health and well-being. Although steps are taken to reduce
risks in the workplace, safety is a matter of personal responsibility. A lab
may have several safety hazards that must be known and understood by all
students or employees working in the lab. It is the responsibility of each
person in the lab to know and follow basic laboratory safety rules, to
understand how to safely operate equipment, understand the hazards of
materials they are working with and to work to reduce potential risks. In the
event of an accident, it is critical to know the location and use of emergency
equipment. Having this knowledge should help to prevent accidents and
minimize damage that might occur in the event of an accident.
, BIOL 1409 lab safety and microscope review study guid
exam 2022
Laboratory Safety Guidelines and
Contract General Rules
No guests are allowed in the lab.
Know emergency procedures, use and location of emergency
equipment (emergency exits, fire extinguishers, fire blanket, eye
wash station, first aid kit, and broken glass container).
In case of fire, evacuate the room and assemble outside the building.
Report all accidents, no matter how insignificant they appear, to a
laboratory supervisor
Be aware of your surroundings and potential dangers created by others.
Personal Protection
Do not smoke, eat, drink, chew gum, or apply cosmetics in a laboratory.
Wear Correct use and care of the laboratory equipment is considered a
fundamental part of good laboratory technique. All students working in
the microbiology laboratory are responsible for maintaining equipment
and materials in proper working condition. clothing such as long pants,
closed-toe shoes, a lab coat, and goggles.
Tie long hair up or behind the shoulders. Do not wear long, dangling
jewelry or scarfs.
Dispose of gloves in the laboratory trash. Do not wear lab coats and
gloves in public areas. You will need to dispose of gloves and take off
your lab coat before leaving the lab.
Cover cuts or scrapes with a sterile, waterproof bandage before
entering a lab.
Wash skin immediately and thoroughly if contaminated by chemicals or
microorganisms.
Wash your hands regularly, with soap and water, especially after
working with bacteria.
If you are pregnant or ill, please let your lab instructor know
immediately.
Let your lab instructor know before leaving the classroom.
Handling Chemicals
Keep all containers capped with the appropriate lid. Clearly label
items produced in the lab.
If a chemical is splashed into the eyes or skin, flush for 15 minutes.
Clean up spills and broken glass immediately. Use a broom and
dustpan to pick up broken glass.
Keep chemicals away from direct heat or sunlight. Keep containers
of alcohol, acetone, and other flammable liquids away from flames.
Read labels carefully. Be aware of hazardous chemicals and precautions
for safe use.
Follow instructions about proper disposal of lab reagents.
exam 2022
Objectives:
Develop good laboratory practices.
Properly use Personal Protective Equipment (PPE).
Identify the location and proper use of emergency equipment.
Identify when a stereomicroscope (dissecting microscope) versus
a compound light microscope would be used in the lab
Describe the major differences between light microscopes and electron
microscopes
Identify the parts of a compound light microscope and stereomicroscope
Describe the proper way to carry a microscope
Determine the total magnification of an object being viewed by
a compound light microscope if given the ocular lens and
objective lens magnifications
Executing the techniques to prepare wet mounts of animal and plant
tissues
I. Introduction
The laboratory is a fun place in which we can explore many concepts that we
are learning in the lecture portion of the course. We want to make sure that
every single one of you can explore the different topics in a safe manner. In
this lab you will learn about some lab safety rules that you must follow. Many
of the labs will require to use of microscopes. In this lab you will also learn
correct use and care of the laboratory equipment. All students working in the
laboratory are responsible for maintaining equipment and materials in proper
working conditions. Let us explore!
II. Lab Safety
Laboratory safety involves all the measures taken by the laboratory worker,
laboratory owner, institution, and regulatory agencies to eliminate potential
harm to human health and well-being. Although steps are taken to reduce
risks in the workplace, safety is a matter of personal responsibility. A lab
may have several safety hazards that must be known and understood by all
students or employees working in the lab. It is the responsibility of each
person in the lab to know and follow basic laboratory safety rules, to
understand how to safely operate equipment, understand the hazards of
materials they are working with and to work to reduce potential risks. In the
event of an accident, it is critical to know the location and use of emergency
equipment. Having this knowledge should help to prevent accidents and
minimize damage that might occur in the event of an accident.
, BIOL 1409 lab safety and microscope review study guid
exam 2022
Laboratory Safety Guidelines and
Contract General Rules
No guests are allowed in the lab.
Know emergency procedures, use and location of emergency
equipment (emergency exits, fire extinguishers, fire blanket, eye
wash station, first aid kit, and broken glass container).
In case of fire, evacuate the room and assemble outside the building.
Report all accidents, no matter how insignificant they appear, to a
laboratory supervisor
Be aware of your surroundings and potential dangers created by others.
Personal Protection
Do not smoke, eat, drink, chew gum, or apply cosmetics in a laboratory.
Wear Correct use and care of the laboratory equipment is considered a
fundamental part of good laboratory technique. All students working in
the microbiology laboratory are responsible for maintaining equipment
and materials in proper working condition. clothing such as long pants,
closed-toe shoes, a lab coat, and goggles.
Tie long hair up or behind the shoulders. Do not wear long, dangling
jewelry or scarfs.
Dispose of gloves in the laboratory trash. Do not wear lab coats and
gloves in public areas. You will need to dispose of gloves and take off
your lab coat before leaving the lab.
Cover cuts or scrapes with a sterile, waterproof bandage before
entering a lab.
Wash skin immediately and thoroughly if contaminated by chemicals or
microorganisms.
Wash your hands regularly, with soap and water, especially after
working with bacteria.
If you are pregnant or ill, please let your lab instructor know
immediately.
Let your lab instructor know before leaving the classroom.
Handling Chemicals
Keep all containers capped with the appropriate lid. Clearly label
items produced in the lab.
If a chemical is splashed into the eyes or skin, flush for 15 minutes.
Clean up spills and broken glass immediately. Use a broom and
dustpan to pick up broken glass.
Keep chemicals away from direct heat or sunlight. Keep containers
of alcohol, acetone, and other flammable liquids away from flames.
Read labels carefully. Be aware of hazardous chemicals and precautions
for safe use.
Follow instructions about proper disposal of lab reagents.
