BIOL 2P03 Name:_________________________
Midterm 1 Student Number:________________
Section 1 (17 marks total). Answer all of the questions below using the Scantrons provided.
1. Which of the following types of microscopy would allow you to see the interior structure
(e.g. the tubular structure of cristae) of mitochondria?
a) Light microscopy
b) Transmission electron microscopy
c) Fluorescence microscopy
d) All of the above
e) None of the above
2. Flow cytometry is a technique that can be used to:
a) Identify cells with particular characteristics from within a cell suspension
b) Separate one type of cell from other types
c) Use fluorescence detection to measure cellular properties or events
d) All of the above
e) None of the above
3. The term ‘senescent’ applied to a cell in culture indicates the cell is:
a) Cancerous
b) No longer capable of replicating
c) Fermenting glucose
d) Actively dividing
4. Which of the following statements about cellular transformation is/are correct?
a) It can occur spontaneously
b) It can be caused by viral infection
c) Transformed cells grow indefinitely
d) All of the above
e) None of the above
5. To rapidly isolate a single cell type from a mixture of multiple cell types, which of the
following techniques could you use?
a) Fluorescence microscopy
b) Magnetic cell sorting
c) Conventional light microscopy
d) All of the above
e) None of the above
, BIOL 2P03 Name:_________________________
Midterm 1 Student Number:________________
6. Which of the following molecules could pass through a phospholipid bilayer at a
biologically useful rate without the assistance of a transporter or channel?
a) O2
b) N2
c) Fatty acid
d) All of the above
e) None of the above
7. Which of the following are constituents of a biological membrane?
a) Sphingolipids
b) Dipalmitoylphosphatidylcholine
c) Cholesterol
d) All of the above
e) None of the above
8. What type of membrane protein is the Na+/K+ ATPase?
a) Peripheral
b) Lipid-anchored
c) Integral
d) All of the above
e) None of the above
9. An annular lipid is
a) Only present at certain times of year
b) Found in inverse hexagonal phase structures
c) Associated with specific membrane proteins
d) All of the above
e) None of the above
10. Cardiolipin is a unique phospholipid with four fatty acyl chains. Which of the following
statements about cardiolipin is/are correct?
a) It is present only in the nuclear envelope
b) It interacts with cytochrome c and plays a role in apoptosis
c) It is made from cholesterol
d) All of the above
e) None of the above
11. Which of the following is/are a step in the synthesis of phosphatidylcholine?
a) Synthesis of fatty acids from acetyl CoA
b) Esterification of fatty acids to glycerol
c) Addition of phosphocholine to the phosphatidic acid
d) All of the above
e) None of the above
Midterm 1 Student Number:________________
Section 1 (17 marks total). Answer all of the questions below using the Scantrons provided.
1. Which of the following types of microscopy would allow you to see the interior structure
(e.g. the tubular structure of cristae) of mitochondria?
a) Light microscopy
b) Transmission electron microscopy
c) Fluorescence microscopy
d) All of the above
e) None of the above
2. Flow cytometry is a technique that can be used to:
a) Identify cells with particular characteristics from within a cell suspension
b) Separate one type of cell from other types
c) Use fluorescence detection to measure cellular properties or events
d) All of the above
e) None of the above
3. The term ‘senescent’ applied to a cell in culture indicates the cell is:
a) Cancerous
b) No longer capable of replicating
c) Fermenting glucose
d) Actively dividing
4. Which of the following statements about cellular transformation is/are correct?
a) It can occur spontaneously
b) It can be caused by viral infection
c) Transformed cells grow indefinitely
d) All of the above
e) None of the above
5. To rapidly isolate a single cell type from a mixture of multiple cell types, which of the
following techniques could you use?
a) Fluorescence microscopy
b) Magnetic cell sorting
c) Conventional light microscopy
d) All of the above
e) None of the above
, BIOL 2P03 Name:_________________________
Midterm 1 Student Number:________________
6. Which of the following molecules could pass through a phospholipid bilayer at a
biologically useful rate without the assistance of a transporter or channel?
a) O2
b) N2
c) Fatty acid
d) All of the above
e) None of the above
7. Which of the following are constituents of a biological membrane?
a) Sphingolipids
b) Dipalmitoylphosphatidylcholine
c) Cholesterol
d) All of the above
e) None of the above
8. What type of membrane protein is the Na+/K+ ATPase?
a) Peripheral
b) Lipid-anchored
c) Integral
d) All of the above
e) None of the above
9. An annular lipid is
a) Only present at certain times of year
b) Found in inverse hexagonal phase structures
c) Associated with specific membrane proteins
d) All of the above
e) None of the above
10. Cardiolipin is a unique phospholipid with four fatty acyl chains. Which of the following
statements about cardiolipin is/are correct?
a) It is present only in the nuclear envelope
b) It interacts with cytochrome c and plays a role in apoptosis
c) It is made from cholesterol
d) All of the above
e) None of the above
11. Which of the following is/are a step in the synthesis of phosphatidylcholine?
a) Synthesis of fatty acids from acetyl CoA
b) Esterification of fatty acids to glycerol
c) Addition of phosphocholine to the phosphatidic acid
d) All of the above
e) None of the above
