Summary Important Points for Biochemistry

Various Important Tests to detect biochemical substances in serum/ urine:


Substance Test/ Method

Glucose (urine) 1. Dipstick test- Glucose oxidase
2. Benedict’s test



Glucose (serum) 1. GOD-POD
2. HK
3. Folin-Wu Method
4. Nelson-Somogyi method
5. King- Asatoor method
6. O- toludiene method
Fructose (urine) Seliwanoff’s test




Lactose (urine) Methyl amine test




Penrose (urine) Bial’s test




Urinary reducing sugars 1. Benedict’s test
2. Osazone test



Urinary ketone bodies 1. Rothera’s test
2. Gerhardt’s test



Serum bilirubin van den Bergh reaction




Urinary bile pigments 1. Fouchet’s test
2. Gmelin test