DIFFUSION EXPERIMENT
[2022F:BIOL 113L-06] Lab Report 1
Introduction: Diffusion is defined as the net arbitrary movement of particles from the area of
higher concentration to area of lower concentration till the equilibrium is achieved. Several
factors affect the rate of diffusion of particles. It has direct relation to: Concentration Gradient,
Pressure and Temperature and inverse relation to: Resistance and Molecular Weight. Hydrophilic
compounds have more rate of diffusion in hydrophilic solvents than hydrophobic compounds.
This diffusion experiment focuses on the effect of molecular weight and type of solute on rate of
diffusion. Methylene blue and potassium permanganate are to be added to agar petri dish.
Methylene Blue is hydrophobic having molecular weight higher than potassium permanganate
which is hydrophilic. Agar is hydrophilic. So, this can be hypothesized that, potassium
permanganate has more rate of diffusion in agar than methylene blue.
Methodology: In this experiment the aim is to find the association between molecular weight
of the compound and the rate of diffusion. Agar petri dish is to be taken in this experiment and
both methylene blue and potassium permanganate are added to it. Methylene Blue is
hydrophobic compound having molecular weight higher than potassium permanganate which is
hydrophilic compound. Agar is hydrophilic. The rate of diffusion for both compounds is to be
noted.
Results: At 0 minutes, methylene blue colored 2cm of area while potassium permanganate
colored 1cm of area. At 30 minutes, methylene blue colored 4cm of area while potassium
permanganate colored 3cm of area. At 60 minutes, methylene blue colored 4cm of area while
potassium permanganate colored 4cm of area. At 90 minutes, methylene blue colored 4.6cm of
area while potassium permanganate colored 5cm of area.
[2022F:BIOL 113L-06] Lab Report 1
Introduction: Diffusion is defined as the net arbitrary movement of particles from the area of
higher concentration to area of lower concentration till the equilibrium is achieved. Several
factors affect the rate of diffusion of particles. It has direct relation to: Concentration Gradient,
Pressure and Temperature and inverse relation to: Resistance and Molecular Weight. Hydrophilic
compounds have more rate of diffusion in hydrophilic solvents than hydrophobic compounds.
This diffusion experiment focuses on the effect of molecular weight and type of solute on rate of
diffusion. Methylene blue and potassium permanganate are to be added to agar petri dish.
Methylene Blue is hydrophobic having molecular weight higher than potassium permanganate
which is hydrophilic. Agar is hydrophilic. So, this can be hypothesized that, potassium
permanganate has more rate of diffusion in agar than methylene blue.
Methodology: In this experiment the aim is to find the association between molecular weight
of the compound and the rate of diffusion. Agar petri dish is to be taken in this experiment and
both methylene blue and potassium permanganate are added to it. Methylene Blue is
hydrophobic compound having molecular weight higher than potassium permanganate which is
hydrophilic compound. Agar is hydrophilic. The rate of diffusion for both compounds is to be
noted.
Results: At 0 minutes, methylene blue colored 2cm of area while potassium permanganate
colored 1cm of area. At 30 minutes, methylene blue colored 4cm of area while potassium
permanganate colored 3cm of area. At 60 minutes, methylene blue colored 4cm of area while
potassium permanganate colored 4cm of area. At 90 minutes, methylene blue colored 4.6cm of
area while potassium permanganate colored 5cm of area.
