ASCP Exam Practice 2023/173
Questions and Answers
Which is required to generate cDNA
A. DNA polymerase
B. RNase H
C. Restriction Enzyme
D. Helicase - -B
-What is the function of Taq Polymerase - -recognize dsDNA
-You have a 50 μL primer stock at 9pM/μl and want dilute the
stock to 900nM. How much stock should you add(what is the
dilution factor) - -2
-What gene causes hypersensitivity to Irinotecan? - -UGT1A
-Which allele causes hypersensitivity to Carbamazepine? - -HLA-
b*1502
-What gives MLPA its specificity - -Stuffer Sequence
-How is copy number determined? - -MLPA
-What is the gene that cause resistance to Vancomycin - -vanA
-Which assay is FISH best used for - -Prader Willi
-RNA polymerase II: where is it and what does it do - -
Eukaryotic: It catalyzes the transcription of DNA to synthesize
precursors of mRNA and most snRNA and microRNA.
(transcribes mRNA)
-Hazard that is blue and is level 4 what does it mean - -big
health hazard
-Dr calls wanting test results but results have not been signed
out by a medical director what do you do - -don't give out the
result until it has been signed out my the MD
,-What trinucleotide repeat is involved in Fragile X? - -CGG
-In Fragile X, what is considered a full mutation expansion? - -
200+
-What method is used to detect Fragile X? - -Southern blot (now
PCR /mPCR)
-Which gene is the largest and has deletions/duplications as the
most common mutation types - -DMD
-Which HCV geneotype(s) has/have the best prognosis - -
Genotypes 2 &3
-Positive attributes of bDNA - -Can be used for both DNA and
RNA
-What type of primer set is best? - -forward primer should
complement DNA strand and reverse primer should mirror and
inversely complement the forward primer
-What are some things that stabilize RNA? - -Inactivated and
minimizing exposure to environmental/endogenous RNases
mRNA: 5'cap
-What is the concentration of DNA with an OD of 2.0 - -100
μg/mL
-Given the following data what can quantifiably be said about
DNA when 260 = 1.4 and 280 = 1.1 - -There is 70ng of pure
DNA
Sample is contaminated with proteins and phenols
-What is the equation for PCR efficiency - -(1+Σ)^n
-You have a gel where the positive control worked, the negative
control worked, patient 1 had a double band, patient 2 was
, positive, and patient 3 had no bands. What would you do? - -
repeat patient 3
-How do you detect for methylation - -Bisulfate sequencing
-MSI (Micro-Satellite Instability) tests for what? - -Mismatch
Repair Genes
-Translocation at t(15, 17) - -Translation at t(15, 17)
-Coumadin can be affect by a mutation in what gene - -
CYP2C19-KVORC1
-What virus interfere with kidney transplant patients - -BK
-You have 2 HIV viral load tests 5 days apart 1 being high on day
one and 1 being low by day 5. What do you do? - -Look into
storage and transport
-You are given a strand of primers. Calculate the Tm - -2˚(AT) +
4˚(GC)
-When doing a multiplex you observe faint bands. What do you
do - -Adjust the annealing temp
-What do you do when you want to optimize a PCR reaction that
generates short PCR fragments? - -Adjust annealing temperature
-Which is caused by a T-cell rearrangement
- Mantle Cell
- Burkits
- Hodkins Lymphoma
- Sezary - -Sezary
-What protects DNA as it is being replicated - -Single Stranded
Binding Protein
-What opens the DNA double helix up - -Helicase
Questions and Answers
Which is required to generate cDNA
A. DNA polymerase
B. RNase H
C. Restriction Enzyme
D. Helicase - -B
-What is the function of Taq Polymerase - -recognize dsDNA
-You have a 50 μL primer stock at 9pM/μl and want dilute the
stock to 900nM. How much stock should you add(what is the
dilution factor) - -2
-What gene causes hypersensitivity to Irinotecan? - -UGT1A
-Which allele causes hypersensitivity to Carbamazepine? - -HLA-
b*1502
-What gives MLPA its specificity - -Stuffer Sequence
-How is copy number determined? - -MLPA
-What is the gene that cause resistance to Vancomycin - -vanA
-Which assay is FISH best used for - -Prader Willi
-RNA polymerase II: where is it and what does it do - -
Eukaryotic: It catalyzes the transcription of DNA to synthesize
precursors of mRNA and most snRNA and microRNA.
(transcribes mRNA)
-Hazard that is blue and is level 4 what does it mean - -big
health hazard
-Dr calls wanting test results but results have not been signed
out by a medical director what do you do - -don't give out the
result until it has been signed out my the MD
,-What trinucleotide repeat is involved in Fragile X? - -CGG
-In Fragile X, what is considered a full mutation expansion? - -
200+
-What method is used to detect Fragile X? - -Southern blot (now
PCR /mPCR)
-Which gene is the largest and has deletions/duplications as the
most common mutation types - -DMD
-Which HCV geneotype(s) has/have the best prognosis - -
Genotypes 2 &3
-Positive attributes of bDNA - -Can be used for both DNA and
RNA
-What type of primer set is best? - -forward primer should
complement DNA strand and reverse primer should mirror and
inversely complement the forward primer
-What are some things that stabilize RNA? - -Inactivated and
minimizing exposure to environmental/endogenous RNases
mRNA: 5'cap
-What is the concentration of DNA with an OD of 2.0 - -100
μg/mL
-Given the following data what can quantifiably be said about
DNA when 260 = 1.4 and 280 = 1.1 - -There is 70ng of pure
DNA
Sample is contaminated with proteins and phenols
-What is the equation for PCR efficiency - -(1+Σ)^n
-You have a gel where the positive control worked, the negative
control worked, patient 1 had a double band, patient 2 was
, positive, and patient 3 had no bands. What would you do? - -
repeat patient 3
-How do you detect for methylation - -Bisulfate sequencing
-MSI (Micro-Satellite Instability) tests for what? - -Mismatch
Repair Genes
-Translocation at t(15, 17) - -Translation at t(15, 17)
-Coumadin can be affect by a mutation in what gene - -
CYP2C19-KVORC1
-What virus interfere with kidney transplant patients - -BK
-You have 2 HIV viral load tests 5 days apart 1 being high on day
one and 1 being low by day 5. What do you do? - -Look into
storage and transport
-You are given a strand of primers. Calculate the Tm - -2˚(AT) +
4˚(GC)
-When doing a multiplex you observe faint bands. What do you
do - -Adjust the annealing temp
-What do you do when you want to optimize a PCR reaction that
generates short PCR fragments? - -Adjust annealing temperature
-Which is caused by a T-cell rearrangement
- Mantle Cell
- Burkits
- Hodkins Lymphoma
- Sezary - -Sezary
-What protects DNA as it is being replicated - -Single Stranded
Binding Protein
-What opens the DNA double helix up - -Helicase
