Essential Cell Biology Chapter 10 Flashcards Questions and Answers 2023

Restriction endonuclease - -cleaves DNA molecules at a specific sequence of bases à molecular scissors, Laboratory tools, wide spectrum of application. They have sticky and blunt ends. -Enzymes Used in DNA Technology - -I. Restriction endonuclease -cleaves DNA molecules at a specific sequence of bases II. DNA ligase -joins DNA strands together III. DNA polymerase -makes DNA from nucleotides -Taq DNA polymerase IV. Reverse transcriptase -generates complementary DNA (cDNA) from an RNA template -Restriction Fragment Length Polymorphism (RFLP) - -•A technique that exploits variations in DNA sequences. -Variations include DNA length & Mutation. -Restriction sites recognized by restriction endonucleases generate DNA fragments with varied length -Almost obsolete, replaced by DNA sequencing