H212 Week 7: Cytology questions and answers 100% correct

H212 Week 7: Cytology questions and answers 100% correct Loose cellular material can be held together to make into a cell block in all of the following EXCEPT a. agar b. albumin c. blood clot d. resin resin A slide of breast fluid has the cells washing off during staining. To help the cells stay on the slide: a. add acetic acid to the fixative b. fix in neutral buffered formalin c. make a direct smear d. use positively charged slides make a direct smear true or false: all Papanicolaou staining is performed regressively false true or false: The use of alcohol as a solvent for Papanicolaou stain dyes allows for the retention of cellular transparency. true Before staining, remove the Carbowax on slides that were fixed with a commercial fixative by soaking the slide in: a. alcohol b. Saccomanno c. saline d. water alcohol The light green in the various EA formulations for Pap staining is very sensitive to: a. temperature b. concentration c. light d. precipitation light A sputum sample was obtained, homogenized, and then centrifuged into a pellet. The best method of smear preparation from the pellet is: a. crosshatch b. crush c. nickel d. pull-apart pull apart The reaction that occurs when a dye stains a tissue structure a color different from the color of the dye solution is called a. polychromatic b. metachromatic c. orthochromatic d. kodachromatic metachromatic To collect as many cells as possible from sparsely cellular urine, the specimen should have which the following techniques applied? a. crush method b. cytocentrifuge c. fine needle aspiration d. liquid-based cytocentrifuge The three major stain solutions of a Papanicolaou stain protocol are: a. hematoxylin, eosin, light green b. hematoxylin, OG6, EA c. OG6, EA, light green d. OG6, hematoxylin, light green hematoxylin, OG6, EA A polychromatic stain is one that: a. achieves different colors by the use of specific differentiation steps b. results in different colors by using one stain solution c. results in different colors by the use of several different stain solutions d. stains tissue a color different from the staining solutions results in different colors by using one stain solution Which of the following nongynecologic specimens deteriorates quickly (within 24 hours), and should be either brought to the lab immediately for cytopreparation or placed in a prefixative solution? a. ascites fluid b. gadtric washings c. pleural fluids d. urine urine metabolically inactive epithelial cells will be what color with the Pap stain? a. blue b. green c. orange d. pink pink Which of the following cells will stain pink with the Pap stain? a. adenocarcinoma cells b. columnar cells c. histiocytes d. superficial squamous cells superficial squamous cells Which of the following cytopreparation techniques could have an immunohistochemical (IHC) stain done on it without having to change IHC protocols? a. cell block b. liquid-based c. Saccomanno fluid d. cytocentrifugation cell block After smears are prepared from the first drops of cellular material from a fine needle aspirate, any residual material can be recovered by rinsing the needle with which of the following? a. Carbowax b. formalin c. methanol d. saline saline Pure methyl green used at a slightly acid pH is considered a specific stain for: a. aldehydes b. polysaccharides c. DNA d. RNA DNA After staining smears with the Pap stain, the cells do not look crisp pink and blue-green, but rather have a dirty blend of colors. This can be corrected in the future by: a. changing the alcohols after the stains more often b. filtering the first dehydrant alcohol daily c. presoaking slides in 95% alcohol for 10 minutes d. decreasing staining time in EA solution changing the alcohols after the stains more often Cell blocks are usually fixed in: a. formalin b. methanol c. ethanol d. acetic acid formalin A smear prepared for cytology examination is obscured by red blood cells. Which of the following can be added to the fixative, to lyse the red blood cells? a. acetic acid b. formaldehyde c. methanol d. polyethylene glycol acetic acid Orange G in the Pap stain will demonstrate which component in epithelial cells? a. mitochondria b. nuclear chromatin c. rough endoplasmic reticulum d. tonofilaments tonofilaments The microscopic examination of individual cells and their morphology is known as: a. cytology b. immunology c. histology d. microbiology cytology In the Papanicolaou stain, the acid protein dye that imparts a keratin and RBCs a yellow-orange color is: a. Ea b. eosin c. hematoxylin d. OG6 OG6 A specimen of cyst fluid was centrifuged. The resultant sediment at the bottom of the tube had cells that were packed very dense. To prevent this in the future: a. decrease the speed b. increase the time c. use 15 mL conical tube d. retain the supernatant decrease the speed A method for obtaining specimens from superficial or deep organs is: a. cytocentrifugation b. fine needle aspiration c. smear preparation d. thin layer technology fine needle aspiration The mordant in the OG-6 counterstain for the Pap procedure is: a. aluminum sulfate b. ferric chloride c. phosphotungstic acid d. picric acid phosphotungstic acid Which of the following is categorized as a gynecologic cytology specimen? a. cerebral spinal fluid b. endocervical cells c. gastric washings d. pericardial fluids endocervical cells