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Homopolymer Tailing

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Homopolymer Tailing is a technique used to purify DNA. DNA is typically purified by running it through an agarose gel, followed by extraction of the DNA from the gel. However, this process can result in the loss of some DNA fragments, particularly those that are shorter than the fragments that are retained. Homopolymer Tailing can be used to overcome this problem by adding a short DNA sequence (a "tail") to the ends of the fragments that are to be purified. The tail is composed of a repeated sequence of DNA, which can be used to identify the fragments that have been retained. This note gives a more detailed explanation.

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,  A homopolymer is a polymer where all the
subunits are same

 Tailing is the adding a series of nucleotides on to
the 3’-OH termini of a double stranded DNA
molecule using enzyme terminal deoxynucleotidyl
transferase of calf thymus

 Inhomopolymer tailing, gene of interest is tailed
with one nucleotide and vector is tailed with an
complementary base

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April 26, 2023
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Dr mrs adedayo
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