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BSC2010 Lab Exam 1 Comprehensive Studyguide.

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BSC2010 Lab Exam 1 Comprehensive Studyguide
 Section I – The Scientific Process
 Laboratory Procedure 1 (A, B, C, D, E)
 Steps to the Scientific Method
 Observation
 Hypothesis
 Experiment
 Revision of Hypothesis
 Conclusion
 Further Experimentation
 Theorize
 Aspects of Scientific Reasoning
 Inductive Reasoning
 Utilizes specific instances to a generalized conclusion
 Deductive Reasoning
 Utilizes general principles that are known to be true to form a conclusion
 Developing a hypothesis
 If, then
 Must be testable/replicable
 Must be realistic
 Developing tests for a Hypothesis
 Independent Variable (Revolves around Hypothesis)
 Dependent Variable
 Control(s)
 Types of Data
 Qualitative Data
 Revolves around numerical data/numbers
 Quantitative Data
 Revolves around visual cues/observations
 Drawing Conclusions
 Conclusions are drawn from the accuracy of experimental outcomes
 Section II – The Microscope and pH and Buffers
 Laboratory Procedure 2 (A, B, C, D, E, F, G, H)
 Identify the parts of a light microscope




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,  Know the rules for protection of a microscope
 Carry the microscope from the base, never from the arm alone
 Clean the eyepiece before and after use
 Glass slides are fragile be careful
 Use the microscope and prepare wet-mount slides
 Take a slide, put the specimen on the slide
 Drop ONE DROP of distilled water on the slide, and put the slide cover on top
 Estimate the size of objects using the microscope

 Compare a Dissecting Microscope and a Light (Compound) Microscope
 Dissecting Microscope
 Dissection Microscopes have two eyepieces, which allows for a 3-D view.
 Light Microscope

 Identify what Magnification of Objective Lens is being used
 Look on the magnification on the objective pieces
 Define Magnification and Resolution
 Magnification
 The level of detail one can see a specimen
 Resolution
 The size of the field of view
 Know the relationship between Numerical Aperture (NA) and Resolution
Value (R)
 The higher the NA value, the lower the R value.


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