BIOS 251 Week 1 Lab: Part C: PowerPhys# 1 -Enzyme Activity (LABORATORY REPORT)

Table 4: Effect of pH on Sucrase Activity Optical Density ph 3 ph 4 ph 5 ph 6 ph 7 ph 8 ph 9 1 0.150 0.323 0.667 0.926 0.824 0.431 0.186 2 0.190 0.321 0.675 0.955 0.810 0.431 0.168 3 0.173 0.293 0.650 0.958 0.776 0.438 0.147 average 0.171 0.312 0.664 0.946 0.803 0.433 0.167 Effect of pH on Sucrase Activity 1. State the optimum pH for sucrase activity and describe how sucrase activity changes at more acidic and more alkaline pH values. The optimum pH for sucrase is pH of 6 with optimal density of 0.933. 2. Was the rate of increase of sucrase activity higher at a pH of 8.5 or a pH of 5.5? pH of 5.5 Table 5: Effect of Temperature on Sucrase Activity Optical Density 10 °C (50 °F) 20 °C (68 °F) 30 °C (86 °F) 40 °C (104 °F) 50 °C (122 °F) 60 °C (140 °F) 70 °C (158 °F) 1 0.008 0.265 0.775 0.926 0.944 0.824 0.627 2 0.005 0.278 0.773 0.949 0.941 0.810 0.633 3 0.010 0.260 0.793 0.952 0.927 0.833 0.633 average 0.008 0.268 0.780 0.942 0.937 0.822 0.631 Effect of Temperature on Sucrase Activity 3. State the optimum temperature for sucrase activity and describe how sucrase activity changes at lower and higher temperatures. 40 degrees Celsius. Sucrase activity decreases with higher temperature. 4. Was sucrase activity higher at 25 °C or 55 °C? 55 degrees C