MBB331 first midterm exam answer_key - Simon Fraser University MBB 331

MBB331 MIDTERM 1 - Spring 2017 ANSWER KEY NAME (PRINT in block letters):____________________________________________ SFU ID: ________________________________________________________________ TA/TUTORIAL DAY:TIME _______________________________________________ Answer all questions in the spaces provided. Keep your answers concise and legible. If your writing is not legible, you will not be given any marks for your answers. Exam total = 45 marks Section 1. Multiple choice and True/False questions (15 marks). Select ONE best answer. 1. The correct 10-base forward PCR primer starting at position 4 on the following sequence [5’-ATGCGATGTAGGGCGGGATGGAGAGATAGAGAGAGTCACAAT-3’] would be: A. 5-GGGATGGCAT B. 5-ATGCGATGTT C. 5-CGATGTAGGG D. 5-ATGCGATGTA E. 5-GCTACATCCC 2. Site-directed mutagenesis is used for which of the following purposes? A. to insert fragments of more than 100 pairs into a specific DNA site B. to induced site-specific recombination C. to make changes to specific bases in DNA D. to remove large segments of a DNA sequence E. to affinity tag fusion proteins 3. The melting temperature of PCR primers may be calculated using the following formula: A. Tm = 2 (#A+T) + 4(#G+C) B. Tm = 2 (#A+T) +3(#G+C) C. Tm = 3 (#A+T) + 2(#G+C) D. Tm = 2 (#A+T) + 2(#G+C) E. Tm = 2 (#A+T) + (#G+C) 4. Which of the following may be used for screening a protein expression library? A. labeled antibodies B. MALDI-TOF C. site-directed mutagenesis D. bridge PCR sequencing E. real-time PCR