MB ASCP Test Molecular Techniques (20-30%) 100% Correct Answers.

MB ASCP Test Molecular Techniques (20-30%) 100% Correct Answers. What are the 3 types of gel electrophoresis -ANSWER Agarose Polyacrylamide Pulse Field What is gel electrophoresis -ANSWER the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis -ANSWER due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DNA will travel in a gel -ANSWER the size of the DNA fragment What factors affect migration of smaples within a gel -ANSWER buffer concentrations gel make up physical factors time electric current What is agarose gel electrophoresis -ANSWER most common gel- polysaccharide extracted from seaweed- concentrations are varied with the size of DNA trying to be separated larger DNA size = lower concentrations of agar (.5%=1%) smaller DNA size = higher concentrations (2%-3%) What is Polyacrylamide gel electrophoresis -ANSWER used for the resolution of very small DNA segments (PAGE). Can detect 1bp differences in segments. Is chemically prepared and catalyzed into the gel Pore size is easily adjusted during gel preparation for custom gels to particular experiments What is Pulse Field Gel Electrophoresis -ANSWER for resolution of ver large pieces of DNA 50k + Along with specific gel and buffer combinations the electrical field is pulsed or alternated in various directions/dimensions to facilitate movement of large pieces Fled inversion gel electrophoresis is the most common PFGE and is achieved by switching the anode and cathode periodically for repeated forward and back movements of the fragments to gain resolutions. Variations occur at 90 deg to normal electron flow, in circular patterns