ASCP MB III: Laboratory Operations Advanced.

ASCP MB III: Laboratory Operations Ae / lavender - ANSWER- tripotassium EDTA (7.5-15% solution) - virology, molecular biology studies light blue - ANSWER- Tri-Sodium Citrate - Prothrombin time (PT), Activated Partial Thromboplastin Time (APTT), Fibrinogen thrombin time and other blood Coagulation tests. green top collection tube - ANSWER- sodium heparin (freeze-dried) - immunology, virology studies yellow top collection tube - ANSWER- Acid citrate dextrose (ACD) - molecular biology studies. Blood Bank studies, HLA Phenotyping, Paternity testing, Tissue typing etc. royal blue - ANSWER- None/ Di-sodium EDTA - Trace Elements like Cu, Zn, etc, Toxicology and Nutrient determination etc. red top collection tube - ANSWER- no additive - chemistry, serum, viral antibody studies grey - ANSWER- sodium fluoride - Blood Sugar testing, Toxicology tests etc. you receive a specimen in a lavender top vacutainer tube (EDTA). Test requires specimen be in a yellow top ACD vacutainer tube. What should you do? - ANSWERfollow lab protocol for accepting and rejecting specimens best way to store RNA for longer than a month? - ANSWERethanol, at -20°C or -80°C peripheral blood - ANSWER- in EDTA, ACD, heparin (some circ). - clotted/hemolyzed blood very rarely acceptable liquid pap - ANSWERused for HPV testing nasopharyngeal swab - ANSWERFluA/B/RSV bone marrow - ANSWER- analyzed with FISH - requires high resolution methods (HLA typing)