Molecular Diagnostics Chapter 5(revised edition).

Molecular Diagnostics Chapter 5(revised edition).What type of restriction enzyme is commonly used in the laboratory? - ANSWERtype II, 4-6 bp recognition sites restriction map - ANSWERdiagram that shows the lengths of fragments between restriction sites in the strand of DNA If you cut a linear fragment of DNA with a restriction enzyme at three sites, how many fragments do you have? What about if you cut a plasmid at three sites - ANSWER4 fragments, 3 fragments How do you perform restriction mapping? - ANSWERCut DNA with restriction enzyme, then cut with two restriction enzymes, then three and so on you can use the size of the fragments to map where the cut sites are located Are restriction enzyme cuts unique to different strands of nucleic acid? - ANSWERyes due to somatic differences in individuals, so fragment analysis can be used for paternity or forensic profiling star activity - ANSWERwhen REs are not always site-specific and cut at an incorrect sequence. This error increases if the reaction proceeds for too long or an incorrect buffer is used or organic solvents like ethanol Restriction Fragment Length Polymorphisms (RFLPs) - ANSWERDifferences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths (DNA segments resulting from treatment with restriction enzymes). CRISPR - ANSWERclustered regularly interspaced short palindromic repeats