Molecular Diagnostics Chapter 11 ORIGINAL VERSION.

Molecular Diagnostics Chapter 11 ORIGINAL VERSION. What are examples of PCR inhibitors? - ANSWERS.hemoglobin, acidic polysacharrides, nitrate, crystals, beta-human chorionic gonadatropin, heparin, lactoferrin, anticoagulants, sodium polyanethol sulfonate, and polyamines STAR - ANSWERS.stool transport and recovery buffer inactivates infectious organisms What is the purpose of a sensitivity control? - ANSWERS.shows lower limit of detection true negative - ANSWERS.Indicates accurately that a disease is not present. homologous extrinsic - ANSWERS.target-derived control with a non-target derived sequence insert, uses same primers, is not a control for dna extraction heterologous extrinsic - ANSWERS.non-target derived controls that are added to each sample prior to dna extraction, controls for extraction and amplification but needs a second set of primers heterologous intrinsic - ANSWERS.non target sequences present in the sample, requires multiplex or two PCRs, controls for amp and extraction, needs to be optimized so that control amp does not interfere with sample amp Validation - ANSWERS.a series of analyte tests using a particular method to establish method characteristics, must be used to test controls, specificity, sensitivity, and reproducibility of the assay Proficiency testing - ANSWERS.Sample tests performed in a clinical laboratory to determine with what degree of accuracy tests are being performed. Testing samples are checked in the same manner as patient specimens. Competency Testing - ANSWERS.This is mandated by CLIA and tests individual technologists LDT - ANSWERS.Laboratory developed test. A subset of in vitro diagnostic devices are designed, manufactured and offered for clinical use by a single laboratory Bacteria involved in respiratory infection? - ANSWERS.Bordetella, Legionella, Mycobacteria, Chlamydia, Streptococcus, M. tuberculosis