IMMU 101 WT1 - Review Questions and Answers 2023
with complete solution
the study of the systems responsible for recognition of foreign bodies
immunology
what blotting protocol would you use when significant DNA changes are noted in
PT diagnosis
southern blot
primary purpose of the immune system
identify self from non-self
true/false: hybridization with a labeled probe of an unknown nucleic acid
sequence is a shared step in blotting RNA and DNA
false
an immediate hypersensitivity reaction is known as
anaphylaxis
true/false: target sequence to be amplified can be unknown in PCR
false
when are innate immunity mechanisms activated
immediately after infection
what analysis can be used for the detection of gene mutations during cancer
development
PCR
a foreign substance that induces an immune response
antigen
what is used to intensify reactions in nephelometry
antigen coated latex particles
antibodies are composed of
glycoproteins
what assay uses conjugated antibody to detect immune reactions on a
microscopic level
direct immunofluorescent assay (DIA)
what immunoglobulin is usually found in body secretions
IgA
mainly in tears, saliva, etc
an enzyme must exhibit extreme specificity to be used for what assay
enzyme immunoassay (EIA)
which immunoglobulin mediates hypersensitivity (allergic) reactions
IgE
3 pros of utilizing chemiluminescence
- excellent sensitivity
- dynamic range
- doesn't require sample radiation
which immunoglobulin has a "Y" shaped structure when viewed under an
electron microscope
, IgG
immunoassays utilize what as labels
- fluorescent substances
- chemiluminescence
- enzymes
4 phases of the primary response (in order)
- lag
- log
- plateau
- decline
true/false: heterogeneous immunoassays are faster and easier to automate than
homogeneous
false
the anamnestic/memory response characterizes what antibody response
secondary antibody response
during which the release of additional IgG occurs
what solution is used to enhance Direct Bacterial Agglutination
sterile saline with free positive ions
binding strength of an antibody to its antigen
avidity
what hormones can produce a false positive in a pregnancy assay
- LH
- TSH
- FSH
initial force of attraction between an antibody and corresponding antigen
affinity
clear supernatant, few free RBCs and medium sized aggregates would be a _____
reaction
2+
if the skin is penetrated, which cell of the immune system responds rapidly
neutrophils
latex is used in many procedures; what common test procedure does NOT utilize
latex
RPR for syphilis
rapid plasma reagin uses antigen coated carbon
lymphoid surface markers serve what purpose
identify and count CD4 and CD8 cells
agglutination quality can vary based on what 3 factors
- pH
- incubation time
- amount of antigen
what tissue is the sole provider of progenitor cells that can develop into
lymphoblasts
bone marrow
with complete solution
the study of the systems responsible for recognition of foreign bodies
immunology
what blotting protocol would you use when significant DNA changes are noted in
PT diagnosis
southern blot
primary purpose of the immune system
identify self from non-self
true/false: hybridization with a labeled probe of an unknown nucleic acid
sequence is a shared step in blotting RNA and DNA
false
an immediate hypersensitivity reaction is known as
anaphylaxis
true/false: target sequence to be amplified can be unknown in PCR
false
when are innate immunity mechanisms activated
immediately after infection
what analysis can be used for the detection of gene mutations during cancer
development
PCR
a foreign substance that induces an immune response
antigen
what is used to intensify reactions in nephelometry
antigen coated latex particles
antibodies are composed of
glycoproteins
what assay uses conjugated antibody to detect immune reactions on a
microscopic level
direct immunofluorescent assay (DIA)
what immunoglobulin is usually found in body secretions
IgA
mainly in tears, saliva, etc
an enzyme must exhibit extreme specificity to be used for what assay
enzyme immunoassay (EIA)
which immunoglobulin mediates hypersensitivity (allergic) reactions
IgE
3 pros of utilizing chemiluminescence
- excellent sensitivity
- dynamic range
- doesn't require sample radiation
which immunoglobulin has a "Y" shaped structure when viewed under an
electron microscope
, IgG
immunoassays utilize what as labels
- fluorescent substances
- chemiluminescence
- enzymes
4 phases of the primary response (in order)
- lag
- log
- plateau
- decline
true/false: heterogeneous immunoassays are faster and easier to automate than
homogeneous
false
the anamnestic/memory response characterizes what antibody response
secondary antibody response
during which the release of additional IgG occurs
what solution is used to enhance Direct Bacterial Agglutination
sterile saline with free positive ions
binding strength of an antibody to its antigen
avidity
what hormones can produce a false positive in a pregnancy assay
- LH
- TSH
- FSH
initial force of attraction between an antibody and corresponding antigen
affinity
clear supernatant, few free RBCs and medium sized aggregates would be a _____
reaction
2+
if the skin is penetrated, which cell of the immune system responds rapidly
neutrophils
latex is used in many procedures; what common test procedure does NOT utilize
latex
RPR for syphilis
rapid plasma reagin uses antigen coated carbon
lymphoid surface markers serve what purpose
identify and count CD4 and CD8 cells
agglutination quality can vary based on what 3 factors
- pH
- incubation time
- amount of antigen
what tissue is the sole provider of progenitor cells that can develop into
lymphoblasts
bone marrow
