Lab 12: ELISA Questions | 25 Questions with 100% Correct Answers | Verified

Why do we need to determine which cell lines produced the highest recombinant FIX? - To reduce the cost of hemophilia treatment. The cell lines produce recombinant FIX and other proteins. Which biomolecule can specifically detect the presence of a certain protein, such as recombinant FX, in a sample full of other proteins? - Immunoglobins In a Direct ELISA, which components are directly attached to the plate? - Antigens (by positive absorption) Which component of Sandwich ELISA is required in pairs? - antibodies in a Competitive ELISA, how would the signal measured by the detector react to an increase of antigen in the sample? - Decrease What type of ELISA is shown in the diagram? - Indirect In this coding step, the capture antibodies are immobilized on the surface of polystyrene microplate wells. What type of force causes the passive absorption of the captured antibody due