Assume that the original growth curve was done using a well-aerated medium. Predict what the
growth curve would look like if the culture was grown under anoxic conditions. Explain your
thinking. Assume that the original growth curve was done using a rich, high nutrient medium
like Luria-Bertani broth. Predict what the growth curve would look if the culture was grown in a
defined, minimal salts medium (with glucose). Explain your thinking.
Solution
1.under anoxic condition no growth will be seen in case of aerobic bacteria. In case of
aerotolerent or facultative anaerobic bacteria growth curve will looks like normal growth curve.
2. At first growth curve will face a degeneration because there may be some auxotrophc mutant
bacteria which can survive on LB broth but can\'t survive in minimal medium. So mutants will
die and curve face a degeneration. But after that the remaining prototroph wild strains would
give a log phase followed by stationary phase or degeneration phase based upon availability of
nutrients.
growth curve would look like if the culture was grown under anoxic conditions. Explain your
thinking. Assume that the original growth curve was done using a rich, high nutrient medium
like Luria-Bertani broth. Predict what the growth curve would look if the culture was grown in a
defined, minimal salts medium (with glucose). Explain your thinking.
Solution
1.under anoxic condition no growth will be seen in case of aerobic bacteria. In case of
aerotolerent or facultative anaerobic bacteria growth curve will looks like normal growth curve.
2. At first growth curve will face a degeneration because there may be some auxotrophc mutant
bacteria which can survive on LB broth but can\'t survive in minimal medium. So mutants will
die and curve face a degeneration. But after that the remaining prototroph wild strains would
give a log phase followed by stationary phase or degeneration phase based upon availability of
nutrients.
