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Exam (elaborations)

10. Examine Figure 17.7. below. What would be the effect on transcrip

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10. Examine Figure 17.7. below. What would be the effect on transcription if a mutation occurred in the gene that encodes GAL3, so that no functional GAL3 was produced? (note: this question is more about understanding the relationship between regulatory components not necessarily Gal3 specifically) GAL4 UAS GAL80 GAL3 Galactose GAL4 GAL80 GAL3 itth Edition Solution In the absence of functional GAL3 gene, inhibition of GAL4 transcription by GAL80 will not be relieved in the presence of galactose. The genes of galactose region will not be expressed and galactose will not be metabolized as a consequence. (For it to be working, GAL4 need to be expressed in order to bind to UAS.)

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10. Examine Figure 17.7. below. What would be the effect on transcription if a mutation
occurred in the gene that encodes GAL3, so that no functional GAL3 was produced? (note: this
question is more about understanding the relationship between regulatory components not
necessarily Gal3 specifically) GAL4 UAS GAL80 GAL3 Galactose GAL4 GAL80 GAL3 itth
Edition


Solution


In the absence of functional GAL3 gene, inhibition of GAL4 transcription by GAL80 will not be
relieved in the presence of galactose. The genes of galactose region will not be expressed and
galactose will not be metabolized as a consequence. (For it to be working, GAL4 need to be
expressed in order to bind to UAS.)
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