Another issue that created problems getting the pathway to run in E.coli is that the PAL reaction
is reversible. In fact, the PAL reaction used industrially to make phenylalanine. In order to fix
this problem, Bang, et al. looked into non-plant PAL. It is known that the bacterial version of this
enzyme is controlled differently and cinnamic acid, in bacteria, is used for another purpose. They
selected the PAL enzyme from Strepiomyces maritimus. They did the kinetics of both the
forward and reverse reaction. Note that the reverse reaction has two substrates, both of which
were assayed below. Figure 5. The double reciprocal plots for PAL, using phenylalanine (graph
A), or cinnamic acid\' ammonia (graph B) as substrates. The equation for the line in plot A is y
480.17x + 20.88. The equations for the lines in plot B: for cinnamic acid, y = 2.7080 times 10^5
x + 4.1667 times 10^2 and for ammonia, y = 2.2917 times 10^6 x + 4.1667 times 10^2. Fill in
the table below for the kinetic parameters for the PAL with the different substrates. Use [E]_t =
10.0 nM and stick to 3.4 significant figures for your calculations.
Solution
more detailed information needed, or please provide more data and information.
research paper or article type of information.
is reversible. In fact, the PAL reaction used industrially to make phenylalanine. In order to fix
this problem, Bang, et al. looked into non-plant PAL. It is known that the bacterial version of this
enzyme is controlled differently and cinnamic acid, in bacteria, is used for another purpose. They
selected the PAL enzyme from Strepiomyces maritimus. They did the kinetics of both the
forward and reverse reaction. Note that the reverse reaction has two substrates, both of which
were assayed below. Figure 5. The double reciprocal plots for PAL, using phenylalanine (graph
A), or cinnamic acid\' ammonia (graph B) as substrates. The equation for the line in plot A is y
480.17x + 20.88. The equations for the lines in plot B: for cinnamic acid, y = 2.7080 times 10^5
x + 4.1667 times 10^2 and for ammonia, y = 2.2917 times 10^6 x + 4.1667 times 10^2. Fill in
the table below for the kinetic parameters for the PAL with the different substrates. Use [E]_t =
10.0 nM and stick to 3.4 significant figures for your calculations.
Solution
more detailed information needed, or please provide more data and information.
research paper or article type of information.
