ASCP recalls 2017-2018 (corrected) updated 2023
questions & answers 2023 (A+ GRADED 100%
VERIFIED)
3 pairs of hooklets in an ovum
Presence of lupus anticoagulant leads to ....
Which is the agent of hand, foot and mouth disease of humans?
Coxsackie A virus
Detection of antigen in urine specimen can be used for which of the following
type of pneumonia infection
a. Fusobacterium
B. Y. Pestis
C. Legionella
D. Mycobacterium TB.
Legionella
Antler hyphae found in ...
Microsporum Audouinii
What grows on chocolate agar
Haemophilus spp.
*requires X and V factor
It could grow around colonies of with Staph. Aureus forming Satellitism as Staph.
Aureus (in addition Neisseria and Pneumococcus) releases NAD AKA factor Factor 5.
Hemin is factor 10.
Double zone of hemolysis and beta lactamase
Clostridium Perfringens
Which increases first after an MI
Myoglobin
myoglobin>cT>CK>AST>LD
(Myglobin rises within 30 min and last for 18-24 hours.
Tropinin I rises 4-10 hrs post MI, peaks at 14-20 hrs and remains elevated for 4-10
days, most specific and last the longest in blood. CKMB rises 6-10 hours, peaks and 24
hrs and last for 2-3 days, least specific. LD1>LD2 slowest to rise after MI.)
Which of the following parasite cause autoinfection in immunocompromised
patients?
Strongyloides Stercoralis
Which of the following causes antibody against TSH?
Graves Disease = TSHR (thyroid stimulating hormone receptors) antibody
causes thryoid hyperactivity and suppression of TSH
normal or increase T3 and T4
decrease TSH
Which of the following causes antibody against Thyroglubulin and Thyroid cells?
,Hashimotos = Anti-thyroglobulin
decrease T3 and T4
increase TSH
What RBC inclusion can be seen on blood smear of a child who accidentally
ingested moth balls?
Heinz Bodies
Which of the following causes decrease HbA1c?
Sickel cell or any chronic Hemolytic Anemia
Which of the following cells releases histamine/heparin?
Eosinophil and Basophil
Which of the following Mycobacteria we can acquire from tap water?
Mycobacterium Gordonae
Which of the following analytes is cofactor for most of 300 enzymes?
Magnesium (Zinc too)
reference range: 1.7-2.4 mg/dl
Which of the following condition is the most common cause of increase anion
gap?
Uremia (Renal failure), Lactic acidosis, Ketoacidosis, Hypernatremia, ingestion of
Methanol, Ethylene Glycol or Salycilate (SLUMPED)
Double zone of hemolysis bacteria... how to confirmation?
positive reverse CAMP test.
Gram negative anaerobes after a Jaw surgery
Veillonella
reduce nitrate to nitrite, does not ferment carbohydrates.
Gram negative, beta hemolytic, oxidase positive organism isolated from wound.
A/A on TSI
Aeromonas
ONPG negative
N. meningitis
Non lactose fermenter (ONPG Negative): Salmonella spp; Shigella spp; Proteus spp;
Providencia spp and Morganella spp do not produce β-galactosidase so can not ferment
lactose.
What requires oil or olive oil
Malassezia furfur (Tinea Versicolor)
Spaghetti and meatballs appearance
Patient receiving blood from mother requires what type of blood
Irradiated blood
relatives might probably have similar HLA which will recognized by the baby's immune
system as same, hence won't be attacked.
,May cause Graft vs Host disease. Hence, Leukocyte need to be irradiated.
Gamma-irradiation of blood components containing viable lymphocytes is very effective
in preventing TA-GVHD. Irradiation is recommended for all Whole Blood, Red Blood
Cell, Platelets, and Granulocyte transfusions to patients at risk.
HTLV- confirmatory test
Western blot
Human T-cell lymphotropic virus (HTLV) was the first human retrovirus discovered.
HTLV belongs to the Retroviridae family in the genus Deltaretrovirus. Retroviruses are
RNA viruses that use an enzyme called reverse transcriptase to produce DNA from
RNA. The DNA is subsequently incorporated into the host's genome.
Positive RPR negative FTA for Syphilis
False positive
Given a mini panel of antibody reactions. The serum is tested against Group 0
RBCs and cord cells. Reacts with all adult cells, no reaction with cord cells. What
antibody?
anti-I because it did not react with cord cells
Antigens Le a and Le b
Is absorbed from the serum onto red cells
A cold agglutinin picture. The first question asked what disease/infection it was
associated with it and second asked what would cause this blood picture
Mycoplasma pneumoniae and
Cold reacting antibodies
Given mother blood type (AB-) and baby type (O+), what do you do next?
Since O blood type is impossible from AB mom, get a new heelstick from baby.
Biggest problem with PCR?
Contamination with nucleotides.
Quantitative (real time) PCR is useful in detecting ...
EBV
What HBV disease marker is found in individuals with a past infection? life time
marker?
Anti-HBc
Positive anti-HBe, anti-HBc IgG, and Anti-HBs indicates what stage of hepatitis?
Immunity to Hep B due to previous infection
Following a throat infection, patient is having kidney problems. What bacteria
causing it?
Streptococcus pyogenes
Patient has walking pneumoniae and is prescribed penicillin. 2 weeks later, still
sick. What happened?
Bacteria has no cell wall
Potassium permanganate in auramine-rhodamine stain for Mycobacterium
Quenching agent
Specimen of choice for rotavirus?
stool
, Took a swab sample from a wound and incubated on three different medias
(including anaerobic media). Nothing grew. What happened?
Swab material inhibited the sample.
Latex agglutination for Staphylococcus Aureus detects what?
Protein A and clumping factor
Different between Staphylococcus aureus and other Staph. Spp?
Staphylococcus Aureus is Coagulase Positive
How to differentiate between Staphylococcus aureus and Micrococcus
Micrococcus arranged in tetrads
Colonies are yellow and none hemolytic on SBA
most importantly Micrococcus is Furazolidone resistant
Burr cells blood picture
uremia
(uremia and liver disease, artifact (alkaline glass effect)
Stomatocytes blood picture
Liver disease
Badly discolored blood picture with very spiky cells. What caused this?
Slide not dry
Looks like dark Burr cells
Iatrogenic anemia is due to what?
excessive blood draws
(Iatrogenic anemia = means lowered hematocrit and hemoglobin count)
What cell type is increased in Infectious mononucleosis?
lymphs
B cells infected
T cells reactive (pictured)
Lupus anticoagulant causes what?
Increased risk of thrombosis
Sample taken from indwelling catheter. Patient isn't on any anticoagulants yet
PTT and TT are way elevated.
Heparin contamination from the catheter
Anti-Thrombin III
It is a Heparin Co-factor
deficiency is associated with thrombosis
In which case is Magesium monitored?
Pre-eclampsia (eclampsia)
(Twitching, cramping, arrhythmias)
Patient taking primidone showing toxicity, but blood levels normal. What do you
do next?
questions & answers 2023 (A+ GRADED 100%
VERIFIED)
3 pairs of hooklets in an ovum
Presence of lupus anticoagulant leads to ....
Which is the agent of hand, foot and mouth disease of humans?
Coxsackie A virus
Detection of antigen in urine specimen can be used for which of the following
type of pneumonia infection
a. Fusobacterium
B. Y. Pestis
C. Legionella
D. Mycobacterium TB.
Legionella
Antler hyphae found in ...
Microsporum Audouinii
What grows on chocolate agar
Haemophilus spp.
*requires X and V factor
It could grow around colonies of with Staph. Aureus forming Satellitism as Staph.
Aureus (in addition Neisseria and Pneumococcus) releases NAD AKA factor Factor 5.
Hemin is factor 10.
Double zone of hemolysis and beta lactamase
Clostridium Perfringens
Which increases first after an MI
Myoglobin
myoglobin>cT>CK>AST>LD
(Myglobin rises within 30 min and last for 18-24 hours.
Tropinin I rises 4-10 hrs post MI, peaks at 14-20 hrs and remains elevated for 4-10
days, most specific and last the longest in blood. CKMB rises 6-10 hours, peaks and 24
hrs and last for 2-3 days, least specific. LD1>LD2 slowest to rise after MI.)
Which of the following parasite cause autoinfection in immunocompromised
patients?
Strongyloides Stercoralis
Which of the following causes antibody against TSH?
Graves Disease = TSHR (thyroid stimulating hormone receptors) antibody
causes thryoid hyperactivity and suppression of TSH
normal or increase T3 and T4
decrease TSH
Which of the following causes antibody against Thyroglubulin and Thyroid cells?
,Hashimotos = Anti-thyroglobulin
decrease T3 and T4
increase TSH
What RBC inclusion can be seen on blood smear of a child who accidentally
ingested moth balls?
Heinz Bodies
Which of the following causes decrease HbA1c?
Sickel cell or any chronic Hemolytic Anemia
Which of the following cells releases histamine/heparin?
Eosinophil and Basophil
Which of the following Mycobacteria we can acquire from tap water?
Mycobacterium Gordonae
Which of the following analytes is cofactor for most of 300 enzymes?
Magnesium (Zinc too)
reference range: 1.7-2.4 mg/dl
Which of the following condition is the most common cause of increase anion
gap?
Uremia (Renal failure), Lactic acidosis, Ketoacidosis, Hypernatremia, ingestion of
Methanol, Ethylene Glycol or Salycilate (SLUMPED)
Double zone of hemolysis bacteria... how to confirmation?
positive reverse CAMP test.
Gram negative anaerobes after a Jaw surgery
Veillonella
reduce nitrate to nitrite, does not ferment carbohydrates.
Gram negative, beta hemolytic, oxidase positive organism isolated from wound.
A/A on TSI
Aeromonas
ONPG negative
N. meningitis
Non lactose fermenter (ONPG Negative): Salmonella spp; Shigella spp; Proteus spp;
Providencia spp and Morganella spp do not produce β-galactosidase so can not ferment
lactose.
What requires oil or olive oil
Malassezia furfur (Tinea Versicolor)
Spaghetti and meatballs appearance
Patient receiving blood from mother requires what type of blood
Irradiated blood
relatives might probably have similar HLA which will recognized by the baby's immune
system as same, hence won't be attacked.
,May cause Graft vs Host disease. Hence, Leukocyte need to be irradiated.
Gamma-irradiation of blood components containing viable lymphocytes is very effective
in preventing TA-GVHD. Irradiation is recommended for all Whole Blood, Red Blood
Cell, Platelets, and Granulocyte transfusions to patients at risk.
HTLV- confirmatory test
Western blot
Human T-cell lymphotropic virus (HTLV) was the first human retrovirus discovered.
HTLV belongs to the Retroviridae family in the genus Deltaretrovirus. Retroviruses are
RNA viruses that use an enzyme called reverse transcriptase to produce DNA from
RNA. The DNA is subsequently incorporated into the host's genome.
Positive RPR negative FTA for Syphilis
False positive
Given a mini panel of antibody reactions. The serum is tested against Group 0
RBCs and cord cells. Reacts with all adult cells, no reaction with cord cells. What
antibody?
anti-I because it did not react with cord cells
Antigens Le a and Le b
Is absorbed from the serum onto red cells
A cold agglutinin picture. The first question asked what disease/infection it was
associated with it and second asked what would cause this blood picture
Mycoplasma pneumoniae and
Cold reacting antibodies
Given mother blood type (AB-) and baby type (O+), what do you do next?
Since O blood type is impossible from AB mom, get a new heelstick from baby.
Biggest problem with PCR?
Contamination with nucleotides.
Quantitative (real time) PCR is useful in detecting ...
EBV
What HBV disease marker is found in individuals with a past infection? life time
marker?
Anti-HBc
Positive anti-HBe, anti-HBc IgG, and Anti-HBs indicates what stage of hepatitis?
Immunity to Hep B due to previous infection
Following a throat infection, patient is having kidney problems. What bacteria
causing it?
Streptococcus pyogenes
Patient has walking pneumoniae and is prescribed penicillin. 2 weeks later, still
sick. What happened?
Bacteria has no cell wall
Potassium permanganate in auramine-rhodamine stain for Mycobacterium
Quenching agent
Specimen of choice for rotavirus?
stool
, Took a swab sample from a wound and incubated on three different medias
(including anaerobic media). Nothing grew. What happened?
Swab material inhibited the sample.
Latex agglutination for Staphylococcus Aureus detects what?
Protein A and clumping factor
Different between Staphylococcus aureus and other Staph. Spp?
Staphylococcus Aureus is Coagulase Positive
How to differentiate between Staphylococcus aureus and Micrococcus
Micrococcus arranged in tetrads
Colonies are yellow and none hemolytic on SBA
most importantly Micrococcus is Furazolidone resistant
Burr cells blood picture
uremia
(uremia and liver disease, artifact (alkaline glass effect)
Stomatocytes blood picture
Liver disease
Badly discolored blood picture with very spiky cells. What caused this?
Slide not dry
Looks like dark Burr cells
Iatrogenic anemia is due to what?
excessive blood draws
(Iatrogenic anemia = means lowered hematocrit and hemoglobin count)
What cell type is increased in Infectious mononucleosis?
lymphs
B cells infected
T cells reactive (pictured)
Lupus anticoagulant causes what?
Increased risk of thrombosis
Sample taken from indwelling catheter. Patient isn't on any anticoagulants yet
PTT and TT are way elevated.
Heparin contamination from the catheter
Anti-Thrombin III
It is a Heparin Co-factor
deficiency is associated with thrombosis
In which case is Magesium monitored?
Pre-eclampsia (eclampsia)
(Twitching, cramping, arrhythmias)
Patient taking primidone showing toxicity, but blood levels normal. What do you
do next?
