WGU 785 Final Exam Questions And Answers All Verified ( Correct )

WGU 785 Final Exam Questions And Answers All Verified ( Correct ) Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? - ANS His daughters would be carriers. This is x-link recessive. Autosomal: Dominant: - ANS Autosomal: males and females equally affected. Dominant: non-carrier parents polymerase chain reaction (PCR) - ANS The process of copying DNA in the lab. Uses Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA primers. 3 Steps of PCR - ANS 1. Denaturation: DNA is heated to 95C to separate it. 2. Annealing: reaction is cooled to 50C; primers stick to the DNA you want to copy and add DNA polymerase. 3. Elongation: reaction heated to 70C and DNA polymerase, adding nucleotides building a new DNA strand. Base Excision Repair (BER) - ANS How you repair a mutation. BER is used to repair damage to a base caused by harmful molecules. You remove the base that is damaged and replace it. *BER removes a single nucleotide* DNA glycolsylase - sees damaged DNA and removes it. DNA polymerase-puts the right one back in while DNA ligase seals it. Mismatch repair (MMR) occurs during: - ANS replication. DNA polymerase proofreads but sometimes a mismatch pair gets through. MMR removes a large section of the nucleotides from the new DNA and DNA polymerase tries again. (Ex: C-T instead of C-A) Mismatch Repair corrects what kind of DNA damage? - ANS When a base is mismatched due to errors in replication. Such as G-T instead of G-C. DNA polymerase comes by and fixes it. What happens when DNA polymerase binds to DNA to make RNA? - ANS TRANSCRIPTION! DNA polymerase takes the individual nucleotides and matches them to the parental sequences to ensure a correct pair. It must bind with RNA primer to work. What is needed for DNA replication? - ANS DNA polymerase Nonsense Mutation - ANS Change in 1 nucleotide produces a STOP codon Stop= nonsense because it is no more. Silent Mutation - ANS Change in 1 nucleotide but codes for the same amino acid. Silent= the change doesn't change the name of the protein Missense Mutation - ANS Change in 1 nucleotide leads to a code for a different amino acid. Missense = mistake was made. What happends during RNA splicing? -