Ace ASCP Exam Study Guide 2022 with complete solution
Which of the following condition is the most common cause of increase anion
gap?
A.Metabolic alkalosis
B. Metabolic acidosis
Respiratory acidosis
Respiratory alkalosis
B. Metabolic acidosis
High anion gap metabolic acidosis is caused generally by the body producing too much
acid or not producing enough bicarbonate. This is often due to an increase in lactic acid
or ketoacids, or it may be a sign of kidney failure. More rarely, high anion gap metabolic
acidosis may be caused by ingesting methanol or overdosing on aspirin.
Which of the following enzymes has the longest elevation after Myocardial
Infarction (MI)?
CK-MB
Myoglobin
Troponin
AOTA
C. Troponin
Troponin is elevated up to 10-14 days after Myocardial Infarction.
Lactic acid specimen:
Chilled and separated from cells
Heated
Room temperature
Request EDTA sample only
A. Chilled and separated from cells
Feedback Special Handling: No tourniquet. Place on ice immediately and deliver to lab
or aliquot within 15 minutes. DO NOT FREEZE WHOLE BLOOD SPECIMEN.
Specific gravity measured by refractometer: 1.035. Protein and glucose both trace
in reagent strip:
Presence X-ray medium
Albuminuria
,Glucosuria
Normal
A. Presence X-Ray medium
Feedback Xray medium/Radiographic dye can cause erroneous results in urine specific
gravity measurement.
A patient with walking pneumonia is being treated with penicillin. State one
possible condition why the patient is not recovering:
The organism produces beta lactamase
The organism has no cell wall
The organism has capsule
The organism produces an enzyme that neutralizes the antibiotic
B. The organism has no cell wall.
Feedback Pneumonia caused by Mycoplasma pneumoniae is routinely treated with
antibiotics, although the disease is usually self-limiting. The disease is treated with
macrolide, tetracycline, or fluoroquinolone classes of antibiotics. All mycoplasmas lack a
cell wall and, therefore, all are inherently resistant to beta-lactam antibiotics (e.g.,
penicillin).
What is the coefficient of variation if the mean is 90 and the standard deviation is
5.48?
2.75
6.09
5.68
30
B. 6.09
Feedback:
CV= SD/meanX100,
(5.48/90)X100= 6.09.
Blood smear below denotes Hairy Cell Leukemia (HCL), which of the following
test is used to identify this type of leukemia?
Image. 1
Myeloperoxidase
Tartrate-resistant acid phosphatase
,Tartrate-resistant alkaline phosphatase
Sudan Black
B. Tartrate-resistant acid phosphatase
Feedback:
Hairy cells demonstrate strong positivity for tartrate-resistant acid phosphatase (TRAP)
staining. The hairy cells are larger than normal and positive for CD19, CD20, CD22,
CD11c, CD25, CD103, and FMC7.
Use to differentiate Salmonella from Citrobacter:
Lysine decarboxylase
ONPG
Indole
Vogues Proskauerterm-9
A. Lysine decarboxylase
Feedback:
Salmonella (+); Citrobacter (-)
Arrange the common Rh antigens according to immunogenicity (greatest to
least):
D>c>E>C>e
D>C>E>c>e
D>c>E>e>C
C>D>c>E>e
A. D>c>E>C>e
Feedback Most immunogenic D > c > E > C > e least immunogenic.
Most common error in Polymerase Chain Reaction (PCR) is NUCLEIC ACID
CONTAMINATION.
A True (Correct Answer)
B False
A. True
What disease is associated with the possession of HLA-B27?
Ankylosing spondylitis
Hashimoto's Disease
, Goodpasture's syndrome
SLE
A. Ankylosing spondylitis
Feedback:
Ankylosing spondylitis belongs to a group of arthritis conditions that tend to cause
chronic inflammation of the spine (spondyloarthropathies). The HLA-B27 gene can be
detected in the blood of most patients with ankylosing spondylitis.
Burr cells are seen in the peripheral blood smear, this is indicative of? (Fig. 2)
Uremia (Kidney Disease)
Anemia
Leukemia
Liver Disease
A. Uremia (kidney Disease)
Feedback:
The above shown picture illustrates Burr cells, these cells are indicative of Uremia
(kidney disease)
Anti IgG (-); C3d (+): What should the MLS do?
Elution
IAT
DAT
Pre-warm
D. Pre-warm
Feedback Prewarm technique can be used to prevent cold-reactive alloantibodies or
autoantibodies from reacting in the IAT phase. Specifically, prewarm technique prevents
cold antibodies from binding complement at RT (as opposed to 37o C ) and
subsequently being detected by anti-C3 in the IAT by polyspecific AHG serum.
Maximum interval which a recipient sample maybe used for crossmatching if the
patient has been recently transfused, has been pregnant?
2 days
6 days
Which of the following condition is the most common cause of increase anion
gap?
A.Metabolic alkalosis
B. Metabolic acidosis
Respiratory acidosis
Respiratory alkalosis
B. Metabolic acidosis
High anion gap metabolic acidosis is caused generally by the body producing too much
acid or not producing enough bicarbonate. This is often due to an increase in lactic acid
or ketoacids, or it may be a sign of kidney failure. More rarely, high anion gap metabolic
acidosis may be caused by ingesting methanol or overdosing on aspirin.
Which of the following enzymes has the longest elevation after Myocardial
Infarction (MI)?
CK-MB
Myoglobin
Troponin
AOTA
C. Troponin
Troponin is elevated up to 10-14 days after Myocardial Infarction.
Lactic acid specimen:
Chilled and separated from cells
Heated
Room temperature
Request EDTA sample only
A. Chilled and separated from cells
Feedback Special Handling: No tourniquet. Place on ice immediately and deliver to lab
or aliquot within 15 minutes. DO NOT FREEZE WHOLE BLOOD SPECIMEN.
Specific gravity measured by refractometer: 1.035. Protein and glucose both trace
in reagent strip:
Presence X-ray medium
Albuminuria
,Glucosuria
Normal
A. Presence X-Ray medium
Feedback Xray medium/Radiographic dye can cause erroneous results in urine specific
gravity measurement.
A patient with walking pneumonia is being treated with penicillin. State one
possible condition why the patient is not recovering:
The organism produces beta lactamase
The organism has no cell wall
The organism has capsule
The organism produces an enzyme that neutralizes the antibiotic
B. The organism has no cell wall.
Feedback Pneumonia caused by Mycoplasma pneumoniae is routinely treated with
antibiotics, although the disease is usually self-limiting. The disease is treated with
macrolide, tetracycline, or fluoroquinolone classes of antibiotics. All mycoplasmas lack a
cell wall and, therefore, all are inherently resistant to beta-lactam antibiotics (e.g.,
penicillin).
What is the coefficient of variation if the mean is 90 and the standard deviation is
5.48?
2.75
6.09
5.68
30
B. 6.09
Feedback:
CV= SD/meanX100,
(5.48/90)X100= 6.09.
Blood smear below denotes Hairy Cell Leukemia (HCL), which of the following
test is used to identify this type of leukemia?
Image. 1
Myeloperoxidase
Tartrate-resistant acid phosphatase
,Tartrate-resistant alkaline phosphatase
Sudan Black
B. Tartrate-resistant acid phosphatase
Feedback:
Hairy cells demonstrate strong positivity for tartrate-resistant acid phosphatase (TRAP)
staining. The hairy cells are larger than normal and positive for CD19, CD20, CD22,
CD11c, CD25, CD103, and FMC7.
Use to differentiate Salmonella from Citrobacter:
Lysine decarboxylase
ONPG
Indole
Vogues Proskauerterm-9
A. Lysine decarboxylase
Feedback:
Salmonella (+); Citrobacter (-)
Arrange the common Rh antigens according to immunogenicity (greatest to
least):
D>c>E>C>e
D>C>E>c>e
D>c>E>e>C
C>D>c>E>e
A. D>c>E>C>e
Feedback Most immunogenic D > c > E > C > e least immunogenic.
Most common error in Polymerase Chain Reaction (PCR) is NUCLEIC ACID
CONTAMINATION.
A True (Correct Answer)
B False
A. True
What disease is associated with the possession of HLA-B27?
Ankylosing spondylitis
Hashimoto's Disease
, Goodpasture's syndrome
SLE
A. Ankylosing spondylitis
Feedback:
Ankylosing spondylitis belongs to a group of arthritis conditions that tend to cause
chronic inflammation of the spine (spondyloarthropathies). The HLA-B27 gene can be
detected in the blood of most patients with ankylosing spondylitis.
Burr cells are seen in the peripheral blood smear, this is indicative of? (Fig. 2)
Uremia (Kidney Disease)
Anemia
Leukemia
Liver Disease
A. Uremia (kidney Disease)
Feedback:
The above shown picture illustrates Burr cells, these cells are indicative of Uremia
(kidney disease)
Anti IgG (-); C3d (+): What should the MLS do?
Elution
IAT
DAT
Pre-warm
D. Pre-warm
Feedback Prewarm technique can be used to prevent cold-reactive alloantibodies or
autoantibodies from reacting in the IAT phase. Specifically, prewarm technique prevents
cold antibodies from binding complement at RT (as opposed to 37o C ) and
subsequently being detected by anti-C3 in the IAT by polyspecific AHG serum.
Maximum interval which a recipient sample maybe used for crossmatching if the
patient has been recently transfused, has been pregnant?
2 days
6 days
