Clinical chemistry I Review Questions And Correct
Answers 2023/2024, 100% Verified Solution
Accuracy of volumetric pipette
1:1000
A pipette with bulb close to the delivery tip is used for viscous fluids & is called
a:
Ostwald-Folin Pipette
The etched ring on the top of the pipette means:
The last drop is to be blown out after the pipettes drains
Standard solution from which 99.95% of the chemical can be retrieved
Primary standards
When preparing solutions:
A percent solution can be prepared from a volumetric solution
A solution which the molecule of solute in solution are equilibrium with the
excess un dissolved molecules is:
Saturated
pH of Pure water
7.0
pH of 1x10 N solution of hydrochloric acid (HCl)
8
Statistical term that most specifically describe analytical precision
Coefficient of variation
Used to determine quality control of all procedures
Reliability
A procedure of 2 SD variation from a mean value. How many values from a
normal population will the 2 SD include:
95.45%
Material with physical & chemical properties closely resembling the test
specimen & containing concentrations of the substance being measured is
known as:
Control
Optical density according to Beer's law
Directly proportional to the concentration
Difference of serum & plasma is that serum doesn't contain:
Fibrinogen
2 control sera are desirable for each general chemistry procedure. Which of these
combination would be appropriate:
Mean of normal range & abnormal (high)
Sodium fluoride is used in specimen collection to:
Prevent glycolysis
Concentration of oxalate recommended to be used as an anticoagulant
1-2mg/ml blood
EDTA works as anticoagulant by:
Chelating calcium
, Venipuncture site for a routine puncture is commonly cleaned with:
70% alcohol
1 precaution to be followed with a specimen for bilirubin determination is
Protect from light
Substance cannot be preserved by freezing
Lacting dehydrogenase or LDH
Serum ions should be drawn at the same time on successive days to avoid:
Diurnal variation
Proteins that is soluble in water
Albumin
Basic building block of proteins; Amino acids are joined together to form a chain-
like structure through:
Peptide bonding
Elements in proteins that differentiates carbohydrates and lipids
Nitrogen
Amino acid that cannot polarized light
Glycine
Denaturation of proteins
Alteration in tertiary structure
Hemoglobin measurement technique employed for blood donors
Flotation technique of Phillips
Ratio of velocities of light in 2 different media
Refractive index
Most anodic proteins on electrophoresis at pH 8.6
Albumin
When a protein is at its isoelectric point the protein will have
Net zero charge
Svedberg (S) number refers to protein:
Ultracentrifugation
What sedimentation class macroglobulins belong?
19.0S
As urine is heated, a precipitated appears at 60 degrees Celsius & disappears at
100 degrees celsius. The substance present is a:
Bence Jones Proteins (BJP)
Process of separating albumin from globulins using sodium sulfate
Salting out
Conversion of protein nitrogen content to protein content depends in the factor:
6.54
NOT true of the biuret reaction
Relatively free from interference by lipids & hemoglobin
In acute renal failure which non protein nitrogen rises the FASTEST
BUN or Blood Urea Nitrogen
Why is bromcresol purple the preffered indicator for albumin dye binding
technic?
There is less interference from pigment
Why is hemolyzed serum should not be used for total protein?
Answers 2023/2024, 100% Verified Solution
Accuracy of volumetric pipette
1:1000
A pipette with bulb close to the delivery tip is used for viscous fluids & is called
a:
Ostwald-Folin Pipette
The etched ring on the top of the pipette means:
The last drop is to be blown out after the pipettes drains
Standard solution from which 99.95% of the chemical can be retrieved
Primary standards
When preparing solutions:
A percent solution can be prepared from a volumetric solution
A solution which the molecule of solute in solution are equilibrium with the
excess un dissolved molecules is:
Saturated
pH of Pure water
7.0
pH of 1x10 N solution of hydrochloric acid (HCl)
8
Statistical term that most specifically describe analytical precision
Coefficient of variation
Used to determine quality control of all procedures
Reliability
A procedure of 2 SD variation from a mean value. How many values from a
normal population will the 2 SD include:
95.45%
Material with physical & chemical properties closely resembling the test
specimen & containing concentrations of the substance being measured is
known as:
Control
Optical density according to Beer's law
Directly proportional to the concentration
Difference of serum & plasma is that serum doesn't contain:
Fibrinogen
2 control sera are desirable for each general chemistry procedure. Which of these
combination would be appropriate:
Mean of normal range & abnormal (high)
Sodium fluoride is used in specimen collection to:
Prevent glycolysis
Concentration of oxalate recommended to be used as an anticoagulant
1-2mg/ml blood
EDTA works as anticoagulant by:
Chelating calcium
, Venipuncture site for a routine puncture is commonly cleaned with:
70% alcohol
1 precaution to be followed with a specimen for bilirubin determination is
Protect from light
Substance cannot be preserved by freezing
Lacting dehydrogenase or LDH
Serum ions should be drawn at the same time on successive days to avoid:
Diurnal variation
Proteins that is soluble in water
Albumin
Basic building block of proteins; Amino acids are joined together to form a chain-
like structure through:
Peptide bonding
Elements in proteins that differentiates carbohydrates and lipids
Nitrogen
Amino acid that cannot polarized light
Glycine
Denaturation of proteins
Alteration in tertiary structure
Hemoglobin measurement technique employed for blood donors
Flotation technique of Phillips
Ratio of velocities of light in 2 different media
Refractive index
Most anodic proteins on electrophoresis at pH 8.6
Albumin
When a protein is at its isoelectric point the protein will have
Net zero charge
Svedberg (S) number refers to protein:
Ultracentrifugation
What sedimentation class macroglobulins belong?
19.0S
As urine is heated, a precipitated appears at 60 degrees Celsius & disappears at
100 degrees celsius. The substance present is a:
Bence Jones Proteins (BJP)
Process of separating albumin from globulins using sodium sulfate
Salting out
Conversion of protein nitrogen content to protein content depends in the factor:
6.54
NOT true of the biuret reaction
Relatively free from interference by lipids & hemoglobin
In acute renal failure which non protein nitrogen rises the FASTEST
BUN or Blood Urea Nitrogen
Why is bromcresol purple the preffered indicator for albumin dye binding
technic?
There is less interference from pigment
Why is hemolyzed serum should not be used for total protein?
