Ascp MLT Exam Questions & Answers
2023 (A+ Graded 100% Correct)
ACD/CPD/CPD2 - ANSWER 21 days
CPDA-1 - ANSWER 35 days
Infants
HDFN from ABO - ANSWER Spherocytes
1st pregnancy
O mother
Permanent deferral - ANSWER Hepatitis after 11
HIV
T. Cruzi
CJD
1 unit or PRBC - ANSWER Raises hemoglobin 1g and hematocrit 3%
Leukoreduced RBCs - ANSWER Less than 5 x 10^6 to prevent febrile non hemolytic
reactions
Cryoprecipitate - ANSWER Factor 8
vWF
Fibrinogen
For DIC
6 hours
Platelets 1unit - ANSWER 5000 10000
Irradiated blood - ANSWER Prevent GVHD
Ulex europaeus - ANSWER Anti H lectin
Dolichos biflorus - ANSWER Anti A1 lectin
Amount of H greatest to least - ANSWER O
,A2
B
A1
A1B
Most immunogenicity antigen - ANSWER D
Weak D - ANSWER Negative immediate spin and positive AHG
Weak D donor - ANSWER Must be labeled Rh positive
Weak D patient - ANSWER Receives D negative
D control - ANSWER AB positive
Any D negative
IgM antibodies - ANSWER I
H
MN
P1
Lewis
IgG antibodies - ANSWER Rh group
K
Duffy
Jk
I, i antibody - ANSWER i converts to I as infant matures
Destroyed by enzymes - ANSWER MN
S
Duffy
Enhanced by enzymes - ANSWER Rh
Kids
Lewis
,I
P1
Dosage - ANSWER MNS
Rh
Kids
Duffy
Top three acute/immediate transfusion reactions and mortality - ANSWER TRALI,
hemolytic transfusion reactions and TACO
IgE antibodies - ANSWER Mild Allergic reactions
IgA antibodies - ANSWER Severe transfusion allergic reaction
Anaphylactic
Positive hemolysis with negative DAT - ANSWER Sickle cell crisis
Thalassemia/G6PD deficient
Unit overheated or frozen
All cells hemolysis
Kernicterus - ANSWER Excess bilirubin in newborn
HDFN from Rh - ANSWER Retics
DAT positive
Immediate jaundice
After first baby
Rosette - ANSWER Screening for fetal hemorrhage
Kleihauer Betke - ANSWER Quantitative fetal maternal bleed
Primary immune response - ANSWER IgM
Secondary immune response - ANSWER IgG
Type 1 hypersensitivity - ANSWER Anaphylactic
Immediate
Type 2 hypersensitivity - ANSWER Antibody depending cytotoxicity
, Transfusion reactions
Hashimotos
Good pasture
Type 3 hypersensitivity - ANSWER Immune complex
Rheumatoid arthritis
SLE
Type 4 hyper sensitivity - ANSWER Delayed
Monocytes and lymphocytes
Ouchterlony - ANSWER Antibodies added to pre-cut wells in center of agar plate and
patient Sera and standards are alternated in wells surrounding the center well
EIA/ELISA - ANSWER Sandwich technique
HCG
Nephelometry - ANSWER Insoluble complexes
Why is pass-through suspension scattered light absorbance is proportional to the
number of insoluble complexes compared to standards
Antibody concentration
Immunofluorescence direct - ANSWER Add florescence labeled anti-body to patient
tissue wash and examined under fluorescent microscope
Immunofluorescence indirect - ANSWER Add patient serum to reagent wash add
florescence label to anti-globulin wash and examined under microscope
FPIA (Fluorescence Polarization Immunoassay) - ANSWER Add reagent antibody and
fluorescent tact antigen to patient serum
Increase polarize light as a negative test decrease polarized light as a positive test
Sensitivity - ANSWER TP/ TP + FN x 100
Specificity - ANSWER TN / TN + FP x 100
Non lattice - ANSWER More sensitive immunoassays nephelometry
Lattice - ANSWER Less sensitive
C reactive protein - ANSWER Acute phase protein
Inflammation
2023 (A+ Graded 100% Correct)
ACD/CPD/CPD2 - ANSWER 21 days
CPDA-1 - ANSWER 35 days
Infants
HDFN from ABO - ANSWER Spherocytes
1st pregnancy
O mother
Permanent deferral - ANSWER Hepatitis after 11
HIV
T. Cruzi
CJD
1 unit or PRBC - ANSWER Raises hemoglobin 1g and hematocrit 3%
Leukoreduced RBCs - ANSWER Less than 5 x 10^6 to prevent febrile non hemolytic
reactions
Cryoprecipitate - ANSWER Factor 8
vWF
Fibrinogen
For DIC
6 hours
Platelets 1unit - ANSWER 5000 10000
Irradiated blood - ANSWER Prevent GVHD
Ulex europaeus - ANSWER Anti H lectin
Dolichos biflorus - ANSWER Anti A1 lectin
Amount of H greatest to least - ANSWER O
,A2
B
A1
A1B
Most immunogenicity antigen - ANSWER D
Weak D - ANSWER Negative immediate spin and positive AHG
Weak D donor - ANSWER Must be labeled Rh positive
Weak D patient - ANSWER Receives D negative
D control - ANSWER AB positive
Any D negative
IgM antibodies - ANSWER I
H
MN
P1
Lewis
IgG antibodies - ANSWER Rh group
K
Duffy
Jk
I, i antibody - ANSWER i converts to I as infant matures
Destroyed by enzymes - ANSWER MN
S
Duffy
Enhanced by enzymes - ANSWER Rh
Kids
Lewis
,I
P1
Dosage - ANSWER MNS
Rh
Kids
Duffy
Top three acute/immediate transfusion reactions and mortality - ANSWER TRALI,
hemolytic transfusion reactions and TACO
IgE antibodies - ANSWER Mild Allergic reactions
IgA antibodies - ANSWER Severe transfusion allergic reaction
Anaphylactic
Positive hemolysis with negative DAT - ANSWER Sickle cell crisis
Thalassemia/G6PD deficient
Unit overheated or frozen
All cells hemolysis
Kernicterus - ANSWER Excess bilirubin in newborn
HDFN from Rh - ANSWER Retics
DAT positive
Immediate jaundice
After first baby
Rosette - ANSWER Screening for fetal hemorrhage
Kleihauer Betke - ANSWER Quantitative fetal maternal bleed
Primary immune response - ANSWER IgM
Secondary immune response - ANSWER IgG
Type 1 hypersensitivity - ANSWER Anaphylactic
Immediate
Type 2 hypersensitivity - ANSWER Antibody depending cytotoxicity
, Transfusion reactions
Hashimotos
Good pasture
Type 3 hypersensitivity - ANSWER Immune complex
Rheumatoid arthritis
SLE
Type 4 hyper sensitivity - ANSWER Delayed
Monocytes and lymphocytes
Ouchterlony - ANSWER Antibodies added to pre-cut wells in center of agar plate and
patient Sera and standards are alternated in wells surrounding the center well
EIA/ELISA - ANSWER Sandwich technique
HCG
Nephelometry - ANSWER Insoluble complexes
Why is pass-through suspension scattered light absorbance is proportional to the
number of insoluble complexes compared to standards
Antibody concentration
Immunofluorescence direct - ANSWER Add florescence labeled anti-body to patient
tissue wash and examined under fluorescent microscope
Immunofluorescence indirect - ANSWER Add patient serum to reagent wash add
florescence label to anti-globulin wash and examined under microscope
FPIA (Fluorescence Polarization Immunoassay) - ANSWER Add reagent antibody and
fluorescent tact antigen to patient serum
Increase polarize light as a negative test decrease polarized light as a positive test
Sensitivity - ANSWER TP/ TP + FN x 100
Specificity - ANSWER TN / TN + FP x 100
Non lattice - ANSWER More sensitive immunoassays nephelometry
Lattice - ANSWER Less sensitive
C reactive protein - ANSWER Acute phase protein
Inflammation
