(MB) ASCP Practice Exam Questions
correctly answered 2022
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - correct answer Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - correct answer
Thymine
What genes would be screened in a breast cancer panel? - correct answer HER2,
ERBB2, BRCA1
Next Generation Sequencing uses: - correct answer Short sequence reads
Purines and pyrimidines differ from each other in that: - correct answer Purines have
two rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answer Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answer 1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not
depend on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answer Nucleotide sequence of the
molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
,Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answer Initiation,
exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answer 3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard PCR indicates that patient has
Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - correct answer 85 CAG repeats in the
Huntington gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - correct answer 16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase - correct answer Helicase
Mutation in what gene is associated with Fragile X syndrome? - correct answer FMR1
Mantle cell lymphoma (MCL) is caused by what translocation? - correct answer t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity": -
correct answer Pol α
Its discovery shed light on why there is simultaneous, though not continuous, synthesis
of DNA on both leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
, Okazaki fragments
Sanger fragments
RNA fragments - correct answer Okazaki fragments
What gene is measured following treatment with imatinib (Gleevec)?
FLT3
BCR/Abl
Jak2
MAPK - correct answer BCR/Abl
What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second - correct answer 50 nucleotides per second
This polymerase is involved in "replicates mitochondrial DNA": - correct answer Pol γ
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntingtin gene. Which of these methods would best accomplish this
task?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR - correct answer Standard PCR
Which of the following storage options is optimal for storing isolated DNA for a period
greater than seven years?
22-25ºC
2-8ºC
-20ºC
-70ºC - correct answer -70ºC
Consider a hypothetical mutation involving gene X. Let's say you amplify a specific
exon, say exon 11, of that gene then you cut it with restriction enzyme W. In a person
without the mutation, cutting the gene with restriction enzyme W generates two
fragments of sizes, 100 bp and 250 bp. Suppose a C>T mutation in gene X deletes a
restriction site, yielding a fragment of 350 bp. You would expect a heterozygous person
for gene X to have these fragments on a restriction gel:
+/+ = 350 bp; 250 bp; 100 bp;
m/+ = Only the 350 bp
m/+ = 350 bp; 250 bp; 100 bp
m/m = 350 bp; 250 bp - correct answer m/+ = 350 bp; 250 bp; 100 bp
correctly answered 2022
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - correct answer Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - correct answer
Thymine
What genes would be screened in a breast cancer panel? - correct answer HER2,
ERBB2, BRCA1
Next Generation Sequencing uses: - correct answer Short sequence reads
Purines and pyrimidines differ from each other in that: - correct answer Purines have
two rings; pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - correct answer Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - correct answer 1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not
depend on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - correct answer Nucleotide sequence of the
molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
,Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - correct answer Initiation,
exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC - correct answer 3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard PCR indicates that patient has
Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - correct answer 85 CAG repeats in the
Huntington gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - correct answer 16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase - correct answer Helicase
Mutation in what gene is associated with Fragile X syndrome? - correct answer FMR1
Mantle cell lymphoma (MCL) is caused by what translocation? - correct answer t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity": -
correct answer Pol α
Its discovery shed light on why there is simultaneous, though not continuous, synthesis
of DNA on both leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
, Okazaki fragments
Sanger fragments
RNA fragments - correct answer Okazaki fragments
What gene is measured following treatment with imatinib (Gleevec)?
FLT3
BCR/Abl
Jak2
MAPK - correct answer BCR/Abl
What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second - correct answer 50 nucleotides per second
This polymerase is involved in "replicates mitochondrial DNA": - correct answer Pol γ
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntingtin gene. Which of these methods would best accomplish this
task?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR - correct answer Standard PCR
Which of the following storage options is optimal for storing isolated DNA for a period
greater than seven years?
22-25ºC
2-8ºC
-20ºC
-70ºC - correct answer -70ºC
Consider a hypothetical mutation involving gene X. Let's say you amplify a specific
exon, say exon 11, of that gene then you cut it with restriction enzyme W. In a person
without the mutation, cutting the gene with restriction enzyme W generates two
fragments of sizes, 100 bp and 250 bp. Suppose a C>T mutation in gene X deletes a
restriction site, yielding a fragment of 350 bp. You would expect a heterozygous person
for gene X to have these fragments on a restriction gel:
+/+ = 350 bp; 250 bp; 100 bp;
m/+ = Only the 350 bp
m/+ = 350 bp; 250 bp; 100 bp
m/m = 350 bp; 250 bp - correct answer m/+ = 350 bp; 250 bp; 100 bp
