MIBO 3510 L exam 1 2023 with 100% correct answers

biosafety level 1 Well-characterized agents which do not cause disease in healthy humans -ex: e. coli BSL2 Moderate potential hazard to humans and the environment (cause mild disease and/or are difficult to contract in the laboratory) -ex: HIV, Zika BSL3 Can cause serious and potentially lethal disease via inhalation -Mycobacterium tuberculosis BSL4 Agents that are easily transmitted via aerosols and cause severe to fatal disease for which there are no available vaccines or treatments -ebola risk group vs biosafety level Risk group: Organism designation Biosafety level: Laboratory protocols -Similar but not completely identical (RG1 organsisms typically require BSL1 precautions, etc.) sterile Meets medical standards to guarantee free of live organisms/viruses aseptic Only contains the organisms you want media source of food for bacteria -liquid, solid, semi-solid pure culture Culture known to contain only one organism (started from 1 cell) culture Collection of live organisms grown in a lab aseptic techniques Sterilize media and containers used for growth of bacteria. Keep cultures, media and containers covered. Sterilize materials such as inoculating loops, pipets, forceps, and saline before touching or handling cultures, media or containers. Do not contaminate yourself, your lab manual or your workspace. Properly discard all cultures, media, materials and containers. sterilization physical methods heat methods -incineration -autoclaving media irradiation methods -ultraviolet light filtration methods -HEPA filters -membrane filters sterilization chemical methods Gas sterilization: - Used for heat sensitive solid materials (plastics) Disinfection/Sanitizing - Application to surfaces Antimicrobials: - Narrow or broad ranges of microbial targets