MIBO 3510 L exam 1 UGA 2023/ 119
Questions and Answers/ Graded A+
biosafety level 1 - -Well-characterized agents which do not cause disease in healthy
humans
-ex: e. coli
-BSL2 - -Moderate potential hazard to humans and the environment (cause mild
disease and/or are difficult to contract in the laboratory)
-ex: HIV, Zika
-BSL3 - -Can cause serious and potentially lethal disease via inhalation
-Mycobacterium tuberculosis
-BSL4 - -Agents that are easily transmitted via aerosols and cause severe to fatal
disease for which there are no available vaccines or treatments
-ebola
-risk group vs biosafety level - -Risk group: Organism designation
Biosafety level: Laboratory protocols
-Similar but not completely identical (RG1 organsisms typically require BSL1
precautions, etc.)
-sterile - -Meets medical standards to guarantee free of live organisms/viruses
-aseptic - -Only contains the organisms you want
-media - -source of food for bacteria
-liquid, solid, semi-solid
-pure culture - -Culture known to contain only one organism (started from 1 cell)
,-culture - -Collection of live organisms grown in a lab
-aseptic techniques - -Sterilize media and containers used for growth of bacteria.
Keep cultures, media and containers covered.
Sterilize materials such as inoculating loops, pipets, forceps, and saline before touching
or handling cultures, media or containers.
Do not contaminate yourself, your lab manual or your workspace.
Properly discard all cultures, media, materials and containers.
-sterilization physical methods - -heat methods
-incineration
-autoclaving media
irradiation methods
-ultraviolet light
filtration methods
-HEPA filters
-membrane filters
-sterilization chemical methods - -Gas sterilization:
- Used for heat sensitive solid materials (plastics)
Disinfection/Sanitizing
- Application to surfaces
Antimicrobials:
, - Narrow or broad ranges of microbial targets
-What causes bacteria to have different morphologies? - --Growth rate (size)
-Motility (rate of spread)
-Pigment production
-Cell wall composition/polysaccharide production
-Media composition
-isolation of pure cultures - -separating cells physically (streak plates) or by dilution
(spread and pour plates)
-streak plate technique - -A small amount of bacteria is spread over the surface of an
agar plate
- not a quantitative method
(spreading tool must be sterilized between streaks)
-Spread and pour plates - -allow enermeration of cells as well as isolation
-> quantitative
Cultures are serially diluted (10-fold sets) before cells are transferred to the agar
-Spread: Diluted culture pipetted on top of agar and spread over surface with a glass rod
-Pour: Diluted culture is pipetted into warm agar and stirred, then poured into a sterile
dish
-What are the 2 energy sources for metabolic classification? - -1. light (photo)
2. chemicals (chemo)
-photo - -energy source is light
-chemo - -energy source is chemicals
Questions and Answers/ Graded A+
biosafety level 1 - -Well-characterized agents which do not cause disease in healthy
humans
-ex: e. coli
-BSL2 - -Moderate potential hazard to humans and the environment (cause mild
disease and/or are difficult to contract in the laboratory)
-ex: HIV, Zika
-BSL3 - -Can cause serious and potentially lethal disease via inhalation
-Mycobacterium tuberculosis
-BSL4 - -Agents that are easily transmitted via aerosols and cause severe to fatal
disease for which there are no available vaccines or treatments
-ebola
-risk group vs biosafety level - -Risk group: Organism designation
Biosafety level: Laboratory protocols
-Similar but not completely identical (RG1 organsisms typically require BSL1
precautions, etc.)
-sterile - -Meets medical standards to guarantee free of live organisms/viruses
-aseptic - -Only contains the organisms you want
-media - -source of food for bacteria
-liquid, solid, semi-solid
-pure culture - -Culture known to contain only one organism (started from 1 cell)
,-culture - -Collection of live organisms grown in a lab
-aseptic techniques - -Sterilize media and containers used for growth of bacteria.
Keep cultures, media and containers covered.
Sterilize materials such as inoculating loops, pipets, forceps, and saline before touching
or handling cultures, media or containers.
Do not contaminate yourself, your lab manual or your workspace.
Properly discard all cultures, media, materials and containers.
-sterilization physical methods - -heat methods
-incineration
-autoclaving media
irradiation methods
-ultraviolet light
filtration methods
-HEPA filters
-membrane filters
-sterilization chemical methods - -Gas sterilization:
- Used for heat sensitive solid materials (plastics)
Disinfection/Sanitizing
- Application to surfaces
Antimicrobials:
, - Narrow or broad ranges of microbial targets
-What causes bacteria to have different morphologies? - --Growth rate (size)
-Motility (rate of spread)
-Pigment production
-Cell wall composition/polysaccharide production
-Media composition
-isolation of pure cultures - -separating cells physically (streak plates) or by dilution
(spread and pour plates)
-streak plate technique - -A small amount of bacteria is spread over the surface of an
agar plate
- not a quantitative method
(spreading tool must be sterilized between streaks)
-Spread and pour plates - -allow enermeration of cells as well as isolation
-> quantitative
Cultures are serially diluted (10-fold sets) before cells are transferred to the agar
-Spread: Diluted culture pipetted on top of agar and spread over surface with a glass rod
-Pour: Diluted culture is pipetted into warm agar and stirred, then poured into a sterile
dish
-What are the 2 energy sources for metabolic classification? - -1. light (photo)
2. chemicals (chemo)
-photo - -energy source is light
-chemo - -energy source is chemicals
