MIBO 3510L final 2023/ 95 Questions with solved
Answers/ 100% Correct.
Which Key elements are required to build carbohydrates?
H, O, P, N, S, C - -H, O C
-Which key nutrients are required to build lipids and phospholipids?
H, P, O, C, N, S - -H, P, O, C, S
-Which key elements are required to build amino acids?
P, S, N H, O, C - -S, N, H, O, C
-Which key elements are required to build nucleic acids?
S, N, H, O, P, C - -N, H, O, P, C
-Explain how a single colony arises on a streak plate and why a culture inoculated from
a single colony is (theoretically) a pure culture. - -a single bacterial cell isolated through
streaking duplicates to yield many genetically identical bacteria
-expected results if you forget to rinse w/ ethanol during gram stain - -all purple cells
under microscope because you don't rinse out crystal violet from gram negative colonies
-expected result if rinse w/ ethanol for too long during gram stain - -all red colonies
because you wash out the crystal violet from gram positive colonies
-expected result if you don't add iodine during gram stain - -all red because the crystal
violet is not fixed into the gram positive colonies and is rinsed out by ethanol
-possible explanation for culture of isolated bacteria w/ gram positive and gram
negative cells - -old culture, gram variable, or impure culture
,-calculate growth rate fora culture that has a cell count of 290,000 cells at 25 hours and
5,560,000 at 33 hours - -0.5327 generations/hr
-Calculate all TDF's for dilution series.
Tube A B C D E
mL trans. 1 1 3 1 1
begin vol. 5 9 7 99 9
IDF
OCDF
volume plated 0.1ml 0.1ml
replica 1 245 33
replica 2 202 28
replica 3 314 25
CFU/ml - -Tube A: 1.67E-01
Tube B: 1.67E-02
Tube C: 5.00E-03
Tube D: 5.00E-05
Tube E: 5.00E-06
CFU/mL: 5.54 E+07
-You have a culture that you estimate contains 5x10^9 cells per mL. You want to plate
three dilutions that should give you a countable number of colonies on the middle
, dilution (plus a safety plate on each side). Which three dilutions should you plate? Plan
on plating 0.1 mL from each dilution
Tube A B C D E F G H I J
ml trans. 1 1 1 1 1 1 1 1 1 1
start ml 9 9 9 9 9 9 9 9 9 9 - -FGH
-Understand differences between Nitrogen Fixation, Ammonification, Nitrification,
Denitrification - -nitrogen fixation: N2 --- NH3 and NH4+
Nitrification: NH4+ --- NO2-/ NO2- --- NO3-
Denitrification: NO3- --- N2
Amonification --- amino acid --- NH3+/NH4+
-When setting up a brand new fish tank, the initial introduction of fish (and their waste)
is typically followed by:
1) spike in ammonia
2) a decrease in ammonia accompanied by a spike in nitrite, and
3) a decrease in nitrite accompanied by an increase in nitrate
explain: - -poop = ammonia which leads to nitrification
-what occurs during the 80 degree step of the enrichment for spore-forming bacteria - -
endospores already present in the soil survive but do not start growing until the
temperature is reduced to 37 degrees
bacteria that can't form endospores are killed
Answers/ 100% Correct.
Which Key elements are required to build carbohydrates?
H, O, P, N, S, C - -H, O C
-Which key nutrients are required to build lipids and phospholipids?
H, P, O, C, N, S - -H, P, O, C, S
-Which key elements are required to build amino acids?
P, S, N H, O, C - -S, N, H, O, C
-Which key elements are required to build nucleic acids?
S, N, H, O, P, C - -N, H, O, P, C
-Explain how a single colony arises on a streak plate and why a culture inoculated from
a single colony is (theoretically) a pure culture. - -a single bacterial cell isolated through
streaking duplicates to yield many genetically identical bacteria
-expected results if you forget to rinse w/ ethanol during gram stain - -all purple cells
under microscope because you don't rinse out crystal violet from gram negative colonies
-expected result if rinse w/ ethanol for too long during gram stain - -all red colonies
because you wash out the crystal violet from gram positive colonies
-expected result if you don't add iodine during gram stain - -all red because the crystal
violet is not fixed into the gram positive colonies and is rinsed out by ethanol
-possible explanation for culture of isolated bacteria w/ gram positive and gram
negative cells - -old culture, gram variable, or impure culture
,-calculate growth rate fora culture that has a cell count of 290,000 cells at 25 hours and
5,560,000 at 33 hours - -0.5327 generations/hr
-Calculate all TDF's for dilution series.
Tube A B C D E
mL trans. 1 1 3 1 1
begin vol. 5 9 7 99 9
IDF
OCDF
volume plated 0.1ml 0.1ml
replica 1 245 33
replica 2 202 28
replica 3 314 25
CFU/ml - -Tube A: 1.67E-01
Tube B: 1.67E-02
Tube C: 5.00E-03
Tube D: 5.00E-05
Tube E: 5.00E-06
CFU/mL: 5.54 E+07
-You have a culture that you estimate contains 5x10^9 cells per mL. You want to plate
three dilutions that should give you a countable number of colonies on the middle
, dilution (plus a safety plate on each side). Which three dilutions should you plate? Plan
on plating 0.1 mL from each dilution
Tube A B C D E F G H I J
ml trans. 1 1 1 1 1 1 1 1 1 1
start ml 9 9 9 9 9 9 9 9 9 9 - -FGH
-Understand differences between Nitrogen Fixation, Ammonification, Nitrification,
Denitrification - -nitrogen fixation: N2 --- NH3 and NH4+
Nitrification: NH4+ --- NO2-/ NO2- --- NO3-
Denitrification: NO3- --- N2
Amonification --- amino acid --- NH3+/NH4+
-When setting up a brand new fish tank, the initial introduction of fish (and their waste)
is typically followed by:
1) spike in ammonia
2) a decrease in ammonia accompanied by a spike in nitrite, and
3) a decrease in nitrite accompanied by an increase in nitrate
explain: - -poop = ammonia which leads to nitrification
-what occurs during the 80 degree step of the enrichment for spore-forming bacteria - -
endospores already present in the soil survive but do not start growing until the
temperature is reduced to 37 degrees
bacteria that can't form endospores are killed
