MIBO 3510 L exam 1 summer 2023/ 51 Questions
and Answers/ Verified/ Graded A+
glucose - -What should you subtract form media if you want to grow
photolithoautotrophs?
-Pseudomonas - -organism that denitrifies and returns N2 to the atmosphere
-rhizobium - -organism in the soil that fixes nitrogen into NH3/NH4+
-nitrosomonas - -organism that oxidizes NH4+ into NO2-
-nitrobacter - -organism that oxidizes NO2- into NO3-
-take out nitrogen - -What should you subtract from media if you want to grow
nitrogen fixing bacteria?
-What is biosafety level 1? - -Well-characterized agents which do not cause disease in
healthy humans
-What is BSL2? - -Moderate potential hazard to humans and the environment (cause
mild disease and/or are difficult to contract in the laboratory)
-What is BSL3? - -Can cause serious and potentially lethal disease via inhalation
-What is BSL4? - -Agents that are easily transmitted via aerosols and cause severe to
fatal disease for which there are no available vaccines or treatments
-sterile - -guaranteed free of live organisms/viruses
-aseptic - -Only contains the organisms you want
, -culture - -Collection of live organisms grown in a lab
-pure culture - -Culture known to contain only one organism (started from 1 cell)
-What causes bacteria to have different morphologies? - --Growth rate (size)
-Motility (rate of spread)
-Pigment production
-Cell wall comp.
-2 energy sources for metabolic classification - -1. light (photo)
2. chemicals (chemo)
-2 electron sources for metabolic classification - -1. organic (organo)
2. inorganic (litho) i.e. NH3,H2
-2 carbon sources for metabolic classification - -1. organic (hetero) i.e. sugars
2. CO2 (auto)
-bacterial media components - --Major nutrients: C, N, P, S + energy source
-Salts for osmotic balance
-May include: trace elements, vitamins
-defined media - -All components (and their concentrations) are known
-complex media - -Biological mixtures/digests added, exact composition unknown
-best for growing a variety of cultural organisms
-fastidious organism - -requires complex nutritional requirements from the
environment
-microaerophile - -requires small amounts of O2
and Answers/ Verified/ Graded A+
glucose - -What should you subtract form media if you want to grow
photolithoautotrophs?
-Pseudomonas - -organism that denitrifies and returns N2 to the atmosphere
-rhizobium - -organism in the soil that fixes nitrogen into NH3/NH4+
-nitrosomonas - -organism that oxidizes NH4+ into NO2-
-nitrobacter - -organism that oxidizes NO2- into NO3-
-take out nitrogen - -What should you subtract from media if you want to grow
nitrogen fixing bacteria?
-What is biosafety level 1? - -Well-characterized agents which do not cause disease in
healthy humans
-What is BSL2? - -Moderate potential hazard to humans and the environment (cause
mild disease and/or are difficult to contract in the laboratory)
-What is BSL3? - -Can cause serious and potentially lethal disease via inhalation
-What is BSL4? - -Agents that are easily transmitted via aerosols and cause severe to
fatal disease for which there are no available vaccines or treatments
-sterile - -guaranteed free of live organisms/viruses
-aseptic - -Only contains the organisms you want
, -culture - -Collection of live organisms grown in a lab
-pure culture - -Culture known to contain only one organism (started from 1 cell)
-What causes bacteria to have different morphologies? - --Growth rate (size)
-Motility (rate of spread)
-Pigment production
-Cell wall comp.
-2 energy sources for metabolic classification - -1. light (photo)
2. chemicals (chemo)
-2 electron sources for metabolic classification - -1. organic (organo)
2. inorganic (litho) i.e. NH3,H2
-2 carbon sources for metabolic classification - -1. organic (hetero) i.e. sugars
2. CO2 (auto)
-bacterial media components - --Major nutrients: C, N, P, S + energy source
-Salts for osmotic balance
-May include: trace elements, vitamins
-defined media - -All components (and their concentrations) are known
-complex media - -Biological mixtures/digests added, exact composition unknown
-best for growing a variety of cultural organisms
-fastidious organism - -requires complex nutritional requirements from the
environment
-microaerophile - -requires small amounts of O2
