BIOCHEM C785 Final Exam Latest update
With Complete Solution
Which mRNA sequence results in a polypeptide that contains three amino acids? -
Correct Answer 5' - CUU GAU ACU UAG - 3'
Which RNA sequence would end in the following polypeptide?
Phe-Thr-Val-Stop - Correct Answer 5' - UUC ACA GUA UAA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3' - Correct Answer 5' - ATT CGA GAT GGC - 3'
Assuming 100% reaction efficiency, how many DNA copies are created after the
completion of four complete PCR cycles? - Correct Answer 16
What is the function of DNA polymerase in the process of PCR? - Correct Answer It
recognizes the primers and uses the available dNTPs to replicate the template DNA
sequence.
5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA
5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA
Which type of mutation and outcome characterizes the Mutant DNA? - Correct Answer
A missense mutation leading to a longer protein
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to
valine (Val).
What change in codons corresponds to this mutation? - Correct Answer GAA to GTA
What are possible blood types of the parents of a patient with blood type AB? - Correct
Answer A and B
Which pair of chromosomes accurately depicts the genotype of a person with an
autosomal recessive mutant HBB allele and sickle cell anemia? - Correct Answer
option 3- 2 mutant genes on chromosome 11
Which pair of chromosomes accurately depicts the genotype of a person with an X-
linked recessive mutant ALAS2 allele and sideroblastic anemia? - Correct Answer
option 3, mutated allele
, Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is
associated with chronic exposure to cocaine and contributes to drug addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to
cocaine increases their expression? - Correct Answer The nucleosomes become more
widely spaced.
Mice with low levels of agouti gene expression tend to have yellow fur and are obese,
while mice with higher levels of expression are brown and of normal weight. Studies
show that expression of the agouti gene in mice can be controlled by epigenetics.
Mice with low levels of agouti gene expression tend to have yellow fur and are obese,
while mice with higher levels of expression are brown and of normal weight. Studies
show that expression of the agouti gene in mice can be controlled by epigenetics.
What scenario describes epigenetic regulation of the agouti gene? - Correct Answer
Mother mice fed methyl-rich diets during pregnancy give birth to offspring with
decreased agouti gene expression compared to those fed a methyl-poor diet.
Oxidative damage to DNA can often result in the alteration of a single nucleotide.
Which DNA repair mechanism would repair this type of damage? - Correct Answer
Base excision repair
Xeroderma pigmentosum (XP) is a recessive genetic disease that occurs when one or
more of the genes that perform nucleotide excision repair are nonfunctional.
Why do XP patients have a much higher incidence of skin cancer than the general
population? - Correct Answer The mutation rate of all other genes is higher due to
failure to repair.
Which reaction is responsible for the formation of polypeptides? - Correct Answer
Dehydration
Which portion of the amino acid is inside the box? - Correct Answer Carboxyl group
Which amino acid side group is polar? - Correct Answer option 2-one with charge
When placed within a polypeptide chain, which amino acid would interact most
favorably with nonpolar amino acids? - Correct Answer option 2-non polar
Which term describes the order of amino acids in a peptide chain? - Correct Answer
Primary structure
When using heat to denature a protein, which force is the first to be disrupted as
temperature increases? - Correct Answer Hydrophobic interactions
With Complete Solution
Which mRNA sequence results in a polypeptide that contains three amino acids? -
Correct Answer 5' - CUU GAU ACU UAG - 3'
Which RNA sequence would end in the following polypeptide?
Phe-Thr-Val-Stop - Correct Answer 5' - UUC ACA GUA UAA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3' - Correct Answer 5' - ATT CGA GAT GGC - 3'
Assuming 100% reaction efficiency, how many DNA copies are created after the
completion of four complete PCR cycles? - Correct Answer 16
What is the function of DNA polymerase in the process of PCR? - Correct Answer It
recognizes the primers and uses the available dNTPs to replicate the template DNA
sequence.
5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA
5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA
Which type of mutation and outcome characterizes the Mutant DNA? - Correct Answer
A missense mutation leading to a longer protein
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to
valine (Val).
What change in codons corresponds to this mutation? - Correct Answer GAA to GTA
What are possible blood types of the parents of a patient with blood type AB? - Correct
Answer A and B
Which pair of chromosomes accurately depicts the genotype of a person with an
autosomal recessive mutant HBB allele and sickle cell anemia? - Correct Answer
option 3- 2 mutant genes on chromosome 11
Which pair of chromosomes accurately depicts the genotype of a person with an X-
linked recessive mutant ALAS2 allele and sideroblastic anemia? - Correct Answer
option 3, mutated allele
, Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is
associated with chronic exposure to cocaine and contributes to drug addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to
cocaine increases their expression? - Correct Answer The nucleosomes become more
widely spaced.
Mice with low levels of agouti gene expression tend to have yellow fur and are obese,
while mice with higher levels of expression are brown and of normal weight. Studies
show that expression of the agouti gene in mice can be controlled by epigenetics.
Mice with low levels of agouti gene expression tend to have yellow fur and are obese,
while mice with higher levels of expression are brown and of normal weight. Studies
show that expression of the agouti gene in mice can be controlled by epigenetics.
What scenario describes epigenetic regulation of the agouti gene? - Correct Answer
Mother mice fed methyl-rich diets during pregnancy give birth to offspring with
decreased agouti gene expression compared to those fed a methyl-poor diet.
Oxidative damage to DNA can often result in the alteration of a single nucleotide.
Which DNA repair mechanism would repair this type of damage? - Correct Answer
Base excision repair
Xeroderma pigmentosum (XP) is a recessive genetic disease that occurs when one or
more of the genes that perform nucleotide excision repair are nonfunctional.
Why do XP patients have a much higher incidence of skin cancer than the general
population? - Correct Answer The mutation rate of all other genes is higher due to
failure to repair.
Which reaction is responsible for the formation of polypeptides? - Correct Answer
Dehydration
Which portion of the amino acid is inside the box? - Correct Answer Carboxyl group
Which amino acid side group is polar? - Correct Answer option 2-one with charge
When placed within a polypeptide chain, which amino acid would interact most
favorably with nonpolar amino acids? - Correct Answer option 2-non polar
Which term describes the order of amino acids in a peptide chain? - Correct Answer
Primary structure
When using heat to denature a protein, which force is the first to be disrupted as
temperature increases? - Correct Answer Hydrophobic interactions
