Avian Adenovirus Virus (AAV)
(HPS/IBH/EDS)
Poultry Pathology(Path-611)
Lecture 10-11
Dr. Muhammad Kashif Saleemi
DVM PhD
Associate Professor (Tenured)
Member NDCC
Department of Pathology, Faculty of Veterinary Science,
University of Agriculture, Faisalabad, Pakistan
, Introduction and History
◼ Adenovirus was first isolated in 1949
◼ DNA Virus and genome is 70-90 nm in diameter and
nonenveloped
◼ Resistant to lipid solvents like ether, chloroform and can withstand
pH range of 3-9
◼ Highly sensitive to formaldehyde
◼ Some strains survive 60 °C and even 70 °C for 30 minutes.
◼ Haemagglutination is a unique feature of FAdV-1 which
hemagglutinates rat erythrocytes between pH6 and 9
◼ Apart from rat erythrocytes agglutination of sheep erythrocytes is
reported for one FAdV-1 strain, indicating some strain variation
, Introduction and History
◼ The adenovirus hexon is the major capsid protein having
type-, group-, and subgroup-specific antigenic determinants
against which antibodies are produced
◼ Group specific determinants are shared by all
aviadenoviruses, but are not present in the siadenoviruses or
atadenoviruses
◼ Restriction enzyme (RE) analysis is used to differentiate 12
recognized serotypes into 5 genotypes designated A–E,
which are now recognized as separate adenovirus species
◼ PCR has been applied for detection of FAdV, with primer
sequences mainly based on the hexon gene
, Introduction and History
◼ Sequencing of variable regions of the hexon gene can be
used to allocate viruses to species A–E
◼ Most chicken isolates have been made in chick kidney (CK)
or chicken embryo liver (CEL) cells.
◼ A chicken hepatoma cell line (LMH cells) also can be used
for isolation and propagation of FAdV
◼ Although it is probable that all aviadenoviruses multiply in the
embryonating egg, not all chicken or turkey isolates cause
recognizable lesions
◼ Aviadenoviruses have been isolated from turkeys, geese,
ducks, and pigeons
(HPS/IBH/EDS)
Poultry Pathology(Path-611)
Lecture 10-11
Dr. Muhammad Kashif Saleemi
DVM PhD
Associate Professor (Tenured)
Member NDCC
Department of Pathology, Faculty of Veterinary Science,
University of Agriculture, Faisalabad, Pakistan
, Introduction and History
◼ Adenovirus was first isolated in 1949
◼ DNA Virus and genome is 70-90 nm in diameter and
nonenveloped
◼ Resistant to lipid solvents like ether, chloroform and can withstand
pH range of 3-9
◼ Highly sensitive to formaldehyde
◼ Some strains survive 60 °C and even 70 °C for 30 minutes.
◼ Haemagglutination is a unique feature of FAdV-1 which
hemagglutinates rat erythrocytes between pH6 and 9
◼ Apart from rat erythrocytes agglutination of sheep erythrocytes is
reported for one FAdV-1 strain, indicating some strain variation
, Introduction and History
◼ The adenovirus hexon is the major capsid protein having
type-, group-, and subgroup-specific antigenic determinants
against which antibodies are produced
◼ Group specific determinants are shared by all
aviadenoviruses, but are not present in the siadenoviruses or
atadenoviruses
◼ Restriction enzyme (RE) analysis is used to differentiate 12
recognized serotypes into 5 genotypes designated A–E,
which are now recognized as separate adenovirus species
◼ PCR has been applied for detection of FAdV, with primer
sequences mainly based on the hexon gene
, Introduction and History
◼ Sequencing of variable regions of the hexon gene can be
used to allocate viruses to species A–E
◼ Most chicken isolates have been made in chick kidney (CK)
or chicken embryo liver (CEL) cells.
◼ A chicken hepatoma cell line (LMH cells) also can be used
for isolation and propagation of FAdV
◼ Although it is probable that all aviadenoviruses multiply in the
embryonating egg, not all chicken or turkey isolates cause
recognizable lesions
◼ Aviadenoviruses have been isolated from turkeys, geese,
ducks, and pigeons
