Bone marrow examination practice questions and answers
2022/2023 with complete solution
At birth where/what type of bone marrow do we have?
all bones contain hematopoietic (red) marrow
At what age does yellow marrow (fat cells) replace red marrow of long bones
(proximal end)
5-7
What type of bone marrow do we have in adulthood?
hematopoietic tissue in axial skeleton and proximal portions of the extremities
What indications are there for bone marrow biopsy?
multilineage abnormalities, circulating blasts, pancytopenia (WBC, RBC all decrease),
diagnose and staging neoplasia
What are the bone marrow specimen collection sites?
posterior superior iliac crest (spine) of the pelvis, anterior superior iliac crest of the
pelvis, sternum, anterior medial surface of the tibia, spinous process of the vertebrae
Why might an aspiration be used?
to identify types and proportions of hematologic cells and to look for morphologic
variance
Why might a core biopsy be used?
for evaluating diseases that characteristically produce focal lesions rather than diffuse
involvement of the marrow.
How do you prepare an aspirate?
collect specimen and blood film within 24 hours, determine the cytologic types and
proportions of hematopoietic cells
How do you prepare a core biopsy?
collect specimen and blood film within 24 hours, fixed in zenker's solution and stained
with H&E, Giemsa, and Prussian Blue. Determine cellularity, anatomic relation of cells
to fat and connective tissue stroma. Evaluate diseases that produce lesions rather than
diffuse involvement of marrow.
What diseases that produce lesions in a core biopsy?
hodgkin lymphoma, non-hodgkin lymphoma, multiple myeloma, metastatic tumor,
amyloid and granulomas
What are some of the dyes used in bone marrow specimen preparation?
Wright, Wright Giemsa, Ferric ferricyanide (prussian blue), myeloperoxidase (MPO),
sudan black B (SBB), periodic acid-Schiff (PAS), esterases, tartrate-resistant acid
phosphatase (TRAP)
What is the use of Prussian blue dye?
detect and estimate marrow storage of iron or iron metabolism abnormalities
Use of MPO?
detects myelocytic cells by staining cytoplasmic granular contents
Use of SBB?
detects myelocytic cells by staining cytoplasmic grnaular contents
Use of PAS?
2022/2023 with complete solution
At birth where/what type of bone marrow do we have?
all bones contain hematopoietic (red) marrow
At what age does yellow marrow (fat cells) replace red marrow of long bones
(proximal end)
5-7
What type of bone marrow do we have in adulthood?
hematopoietic tissue in axial skeleton and proximal portions of the extremities
What indications are there for bone marrow biopsy?
multilineage abnormalities, circulating blasts, pancytopenia (WBC, RBC all decrease),
diagnose and staging neoplasia
What are the bone marrow specimen collection sites?
posterior superior iliac crest (spine) of the pelvis, anterior superior iliac crest of the
pelvis, sternum, anterior medial surface of the tibia, spinous process of the vertebrae
Why might an aspiration be used?
to identify types and proportions of hematologic cells and to look for morphologic
variance
Why might a core biopsy be used?
for evaluating diseases that characteristically produce focal lesions rather than diffuse
involvement of the marrow.
How do you prepare an aspirate?
collect specimen and blood film within 24 hours, determine the cytologic types and
proportions of hematopoietic cells
How do you prepare a core biopsy?
collect specimen and blood film within 24 hours, fixed in zenker's solution and stained
with H&E, Giemsa, and Prussian Blue. Determine cellularity, anatomic relation of cells
to fat and connective tissue stroma. Evaluate diseases that produce lesions rather than
diffuse involvement of marrow.
What diseases that produce lesions in a core biopsy?
hodgkin lymphoma, non-hodgkin lymphoma, multiple myeloma, metastatic tumor,
amyloid and granulomas
What are some of the dyes used in bone marrow specimen preparation?
Wright, Wright Giemsa, Ferric ferricyanide (prussian blue), myeloperoxidase (MPO),
sudan black B (SBB), periodic acid-Schiff (PAS), esterases, tartrate-resistant acid
phosphatase (TRAP)
What is the use of Prussian blue dye?
detect and estimate marrow storage of iron or iron metabolism abnormalities
Use of MPO?
detects myelocytic cells by staining cytoplasmic granular contents
Use of SBB?
detects myelocytic cells by staining cytoplasmic grnaular contents
Use of PAS?
