Microbiology Lab Quiz 2 Questions and correct Answers 2023

Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation. correct answers: Overlapping organisms are hard to identify and diminishes the amount of light that can pass through and makes it difficult to visualize the morphology of single cells. Why is it essential that smears be air-dried? Why can't they be gently heated over a flame to speed up the drying process? correct answers: The integrity of the organisms shape, membrane and other structures can be damaged. Why should you be careful not to overheat the smear during the heat-fixing process? correct answers: so you don't distort the morphology of the organisms. Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? correct answers: It may prevent the organisms from being fixed to the slide and wash away during staining process. It may also interfere with with light reflection under the microscope.