Immunohematology QUESTIONS AND ANSWERS
1. A woman wants to donate blood. Her physical examination reveals the following:
weight-110 lbs, pulse- 73 bpm, blood pressure-125/75 mmHg, Hematocrit- 35%. Which
of the following exclusions applies to the prospective donor?
A. Pulse to high
B. Weight too low
C. Hematocrit too low
D. Blood pressure too low - ANSW- C. Hematocrit too low
Acceptable hematocrit is above 38%
2. A potential donor has no exclusions, but she weights only 95 pounds. What is the
allowable amount of blood (including samples) that can be drawn?
A. 367 mL
B. 378 mL
C. 454 mL
D. 473 mL - ANSW- C. 454 mL
10.5mL/kg
95/2.2=43.2
43.2x10.5= 453.6mL
3. Donors who have received blood or blood products within 12 months of when they
desire to donate are deferred to protect the recipient because the:
A. Blood could have transmitted hepatitis or HIV
B. Blood may have two cell populations
C. Donor May not be able to tolerate the blood loss
D. Donor red cell hemoglobin level may be too low - ANSW- A. Blood could have
transmitted hepatitis or HIV
4. Which of the following conditions would contraindicate autologous pre-surgical
donation?
A. Weight of 100 lbs
B. Age of 14 years
C. Hemoglobin of 12 g/dL
D. Mild bacteremia - ANSW- D. Mild bacteremia
5. Which of the following donors would be deferred indefinitely?
A. History of syphilis
B. History of gonorrhea
,C. Accutane treatment
D Recipient of human growth hormone - ANSW- D Recipient of human growth hormone
6. Which of the following viruses resides exclusively in leukocytes?
A. CMV
B. HIV
C. HBV
D. HCV - ANSW- A. CMV
7. A donor indicates that he has taken two aspirin tablets per day for the last 36 hours.
The unit of blood:
A. May not be used for pooled platelet concentrate preparation
B. Should not be drawn until 36 hours after cesation of aspirin ingestion
C. May be used for pooled platelet concentrate preparation
D. May be used for red blood cells and fresh-frozen plasma production, but the platelets
should be discarded - ANSW- C. May be used for pooled platelet concentrate
preparation
8. Which of the following best describes what must be done with a unit of blood drawn
from a donor who is found to be at high risk of contracting acquired immune deficiency
syndrome (AIDS)?
A. Hold unit in quarantine until donor diagnosis is clarified
B. Use the blood for research dealing with AIDS
C. Properly dispose of unit by autoclaving or incineration
D. Use the plasma and destroy the red blood cells. - ANSW- C. Properly dispose of unit
by autoclaving or incineration
9. Which of the following is least likely to transmit hepatitis?
A. Cryoprecipiate
B. RBC
C. Plasma protein fraction (PPF)
D. Platelets - ANSW- C. Plasma protein fraction (PPF)
10. A pooled sera product from 16 donors has a repeatedly positive nucleic acid test
(NAT) for HCV. The next action that should be taken is to:
A. Permanently exclude all the donors in the pool
B. Test each donor in the pool for HCV
C. Label all the donors as HCV positive
D. Confirm the positive using recombinant immunoblot assay (RIBA) - ANSW- B. Test
each donor in the pool for HCV
11. Allthough cryoprecipitate has primarily been used for treatment of
hypofibrinogenemia and hemophilia A, it contains other blood proteins useful in the
treatment of coagulopathies. Which of the following is not found in cryoprecipitate?
A. Fibronectin
B. Factor XIII
, C. Factor VIII:vW
D. Antithrombin III - ANSW- D. Antithrombin III
12. Even though it is properly collected and stored, which of the following will fresh-
frozen plasma (FFP) not provide?
A. Factor V
B. Factor VIII
C. Factor IX
D. Platelets - ANSW- D. Platelets
13. Blood needs to be prepared for intrauterine transfusion of a fetus with severe HDN.
The red blood cell unit selected is compatible with the mother's serum and has been
leuko-depleted. An additional step that must be taken before transfusion is to:
A. Add pooled platelets and fresh-frozen plasma
B. Check that the RBC group is consistent with the father's
C. Irradiate the RBCs before infusion
D. Test the RBC unit with the neonate's eluate - ANSW- C. Irradiate the RBCs before
infusion
14. The addition of adenine in an anticoagulant-preservative formulation aids in:
A. Maintaining ATP levels for red cell viability
B. Maintaining platelet funciton in stored blood
C. Reducing the plasma K+ levels during storage
D. Maintaining 2,3-PBG levels for oxygen release to the tissue - ANSW- A. Maintaining
ATP levels for red cell viability
15.The pilot tubes for donor unit #3276 break in the centrifuge. You should:
A. Label the blood using the donor's previous records
B. Discard the unit because processing procedures cannot be performed
C. Discard the red cells and salvage the plasma for fractionation
D. Remove sufficient segments to complete donor processing procedures - ANSW- D.
Remove sufficient segments to complete donor processing procedures
A satellite bag containing 250 mL of fresh plasma is selected for quality control of
cryoprecipitate production. Cryoprecipitate is prepared according to standard operating
procedures. The final product has a total volume of 10 mL. The factor VIII assays are I
IU/mL before and 9IU/mL after preparation
16. What is the percent yield of factor VIII in the final cryoprecipitate?
A. 11%
B. 25%
C. 36%
D. 80%
17. Does this product meet AABB standards for cryoprecipitate production?
A. Yes
B. No; the percent recovery is too low
1. A woman wants to donate blood. Her physical examination reveals the following:
weight-110 lbs, pulse- 73 bpm, blood pressure-125/75 mmHg, Hematocrit- 35%. Which
of the following exclusions applies to the prospective donor?
A. Pulse to high
B. Weight too low
C. Hematocrit too low
D. Blood pressure too low - ANSW- C. Hematocrit too low
Acceptable hematocrit is above 38%
2. A potential donor has no exclusions, but she weights only 95 pounds. What is the
allowable amount of blood (including samples) that can be drawn?
A. 367 mL
B. 378 mL
C. 454 mL
D. 473 mL - ANSW- C. 454 mL
10.5mL/kg
95/2.2=43.2
43.2x10.5= 453.6mL
3. Donors who have received blood or blood products within 12 months of when they
desire to donate are deferred to protect the recipient because the:
A. Blood could have transmitted hepatitis or HIV
B. Blood may have two cell populations
C. Donor May not be able to tolerate the blood loss
D. Donor red cell hemoglobin level may be too low - ANSW- A. Blood could have
transmitted hepatitis or HIV
4. Which of the following conditions would contraindicate autologous pre-surgical
donation?
A. Weight of 100 lbs
B. Age of 14 years
C. Hemoglobin of 12 g/dL
D. Mild bacteremia - ANSW- D. Mild bacteremia
5. Which of the following donors would be deferred indefinitely?
A. History of syphilis
B. History of gonorrhea
,C. Accutane treatment
D Recipient of human growth hormone - ANSW- D Recipient of human growth hormone
6. Which of the following viruses resides exclusively in leukocytes?
A. CMV
B. HIV
C. HBV
D. HCV - ANSW- A. CMV
7. A donor indicates that he has taken two aspirin tablets per day for the last 36 hours.
The unit of blood:
A. May not be used for pooled platelet concentrate preparation
B. Should not be drawn until 36 hours after cesation of aspirin ingestion
C. May be used for pooled platelet concentrate preparation
D. May be used for red blood cells and fresh-frozen plasma production, but the platelets
should be discarded - ANSW- C. May be used for pooled platelet concentrate
preparation
8. Which of the following best describes what must be done with a unit of blood drawn
from a donor who is found to be at high risk of contracting acquired immune deficiency
syndrome (AIDS)?
A. Hold unit in quarantine until donor diagnosis is clarified
B. Use the blood for research dealing with AIDS
C. Properly dispose of unit by autoclaving or incineration
D. Use the plasma and destroy the red blood cells. - ANSW- C. Properly dispose of unit
by autoclaving or incineration
9. Which of the following is least likely to transmit hepatitis?
A. Cryoprecipiate
B. RBC
C. Plasma protein fraction (PPF)
D. Platelets - ANSW- C. Plasma protein fraction (PPF)
10. A pooled sera product from 16 donors has a repeatedly positive nucleic acid test
(NAT) for HCV. The next action that should be taken is to:
A. Permanently exclude all the donors in the pool
B. Test each donor in the pool for HCV
C. Label all the donors as HCV positive
D. Confirm the positive using recombinant immunoblot assay (RIBA) - ANSW- B. Test
each donor in the pool for HCV
11. Allthough cryoprecipitate has primarily been used for treatment of
hypofibrinogenemia and hemophilia A, it contains other blood proteins useful in the
treatment of coagulopathies. Which of the following is not found in cryoprecipitate?
A. Fibronectin
B. Factor XIII
, C. Factor VIII:vW
D. Antithrombin III - ANSW- D. Antithrombin III
12. Even though it is properly collected and stored, which of the following will fresh-
frozen plasma (FFP) not provide?
A. Factor V
B. Factor VIII
C. Factor IX
D. Platelets - ANSW- D. Platelets
13. Blood needs to be prepared for intrauterine transfusion of a fetus with severe HDN.
The red blood cell unit selected is compatible with the mother's serum and has been
leuko-depleted. An additional step that must be taken before transfusion is to:
A. Add pooled platelets and fresh-frozen plasma
B. Check that the RBC group is consistent with the father's
C. Irradiate the RBCs before infusion
D. Test the RBC unit with the neonate's eluate - ANSW- C. Irradiate the RBCs before
infusion
14. The addition of adenine in an anticoagulant-preservative formulation aids in:
A. Maintaining ATP levels for red cell viability
B. Maintaining platelet funciton in stored blood
C. Reducing the plasma K+ levels during storage
D. Maintaining 2,3-PBG levels for oxygen release to the tissue - ANSW- A. Maintaining
ATP levels for red cell viability
15.The pilot tubes for donor unit #3276 break in the centrifuge. You should:
A. Label the blood using the donor's previous records
B. Discard the unit because processing procedures cannot be performed
C. Discard the red cells and salvage the plasma for fractionation
D. Remove sufficient segments to complete donor processing procedures - ANSW- D.
Remove sufficient segments to complete donor processing procedures
A satellite bag containing 250 mL of fresh plasma is selected for quality control of
cryoprecipitate production. Cryoprecipitate is prepared according to standard operating
procedures. The final product has a total volume of 10 mL. The factor VIII assays are I
IU/mL before and 9IU/mL after preparation
16. What is the percent yield of factor VIII in the final cryoprecipitate?
A. 11%
B. 25%
C. 36%
D. 80%
17. Does this product meet AABB standards for cryoprecipitate production?
A. Yes
B. No; the percent recovery is too low
