1. Preclinical screening methods of Antihypertensive agents
Introduction
• The animal models of hypertension share many features which are
common to human hypertension.
• Many of these models have been developed by utilizing the etiological
factors that are presumed to be responsible for human hypertension.
Genetic factors.
Hyperactivity of renin-angiotensin- aldosterone system (RAAS) and
Excessive salt intake.
, An ideal animal model of hypertension criteria
• It should be feasible in small animals.
• Simple to perform and uniformly reproducible.
• Should be able to predict the potential antihypertensive properties of
an agent.
• Consume minimal quantities of compounds.
• It should be comparable to some form of human hypertension.
Animals used
• In the past, most studies on experimental hypertension were carried
out on Dogs.
• Currently, rat is the preferred animal species.
• Spontaneous hypertensive rat (SHR), the genetic strain of
hypertensive rat, is the animal of choice.
IN VITRO & EX-VIVO MODELS IN VIVO MODELS
1.Monocrotaline Induced Pulmonary 1.ACE Inhibition in rat
Hypertension
2.ACE Inhibition in GUENIA PIG 2.Goldbalt Hypertension
illeum
3. β1 Sympatholytic Activity in 3.Chronic renal hypertension in rats
GUENIA PIG Atria
4.Tail cuff method
5.Salt- sensitive Dahl Rats
6.Renin inhibition in Monkeys
7.Chronic renal hypertension in Dogs
8.Antihypertensive and vasodilator
action in rats
Introduction
• The animal models of hypertension share many features which are
common to human hypertension.
• Many of these models have been developed by utilizing the etiological
factors that are presumed to be responsible for human hypertension.
Genetic factors.
Hyperactivity of renin-angiotensin- aldosterone system (RAAS) and
Excessive salt intake.
, An ideal animal model of hypertension criteria
• It should be feasible in small animals.
• Simple to perform and uniformly reproducible.
• Should be able to predict the potential antihypertensive properties of
an agent.
• Consume minimal quantities of compounds.
• It should be comparable to some form of human hypertension.
Animals used
• In the past, most studies on experimental hypertension were carried
out on Dogs.
• Currently, rat is the preferred animal species.
• Spontaneous hypertensive rat (SHR), the genetic strain of
hypertensive rat, is the animal of choice.
IN VITRO & EX-VIVO MODELS IN VIVO MODELS
1.Monocrotaline Induced Pulmonary 1.ACE Inhibition in rat
Hypertension
2.ACE Inhibition in GUENIA PIG 2.Goldbalt Hypertension
illeum
3. β1 Sympatholytic Activity in 3.Chronic renal hypertension in rats
GUENIA PIG Atria
4.Tail cuff method
5.Salt- sensitive Dahl Rats
6.Renin inhibition in Monkeys
7.Chronic renal hypertension in Dogs
8.Antihypertensive and vasodilator
action in rats
