VC4
Datum van uitvoering experiment: 15-03-2017 en 22-03-2017
Productie van bio ethanol uit aardappelzetmeel
Summary
The aim of this experiment is to produce bio ethanol. At the beginning of this experiment the
enzyme amylase has been used to convert potato starch to glucose. This glucose has been converted
to ethanol with the use of baker’s yeast. The yield of this part of the experiment is: 14,42 %. This
corresponds to 4,005 grams of glucose. The ethanol concentration has been determined with a GC.
Measuring the weight decrease and the decrease of the glucose concentration is another way to
determine the ethanol concentration. These methods were also used in this experiment. The
spectrophotometer has been used to measure the decrease in glucose concentration. The reagent
Roche Glucose GOD-PAP, R1 has been used to color the analyte. The results of this experiment are
shown in table 1.
Table 1 Results
Reading Device % ethanol Mass ethanol (Gram) % yield
Glucose Spectrophotometer * * *
Ethanol GC * * *
Weight Mass balance * * *
*BESCHIKBAAR IN VOLLEDIGE VERSIE!!
1. Inleiding
1.1 Doel
Het doel van dit experiment is om bio ethanol te produceren. Dit word gedaan door aardappel
zetmeel met behulp van bacteriële amylase en bacteriële amyloglucosidase om te zetten in glucose.
Deze glucose word vervolgens met behulp van bakkersgist omgezet in ethanol.
Datum van uitvoering experiment: 15-03-2017 en 22-03-2017
Productie van bio ethanol uit aardappelzetmeel
Summary
The aim of this experiment is to produce bio ethanol. At the beginning of this experiment the
enzyme amylase has been used to convert potato starch to glucose. This glucose has been converted
to ethanol with the use of baker’s yeast. The yield of this part of the experiment is: 14,42 %. This
corresponds to 4,005 grams of glucose. The ethanol concentration has been determined with a GC.
Measuring the weight decrease and the decrease of the glucose concentration is another way to
determine the ethanol concentration. These methods were also used in this experiment. The
spectrophotometer has been used to measure the decrease in glucose concentration. The reagent
Roche Glucose GOD-PAP, R1 has been used to color the analyte. The results of this experiment are
shown in table 1.
Table 1 Results
Reading Device % ethanol Mass ethanol (Gram) % yield
Glucose Spectrophotometer * * *
Ethanol GC * * *
Weight Mass balance * * *
*BESCHIKBAAR IN VOLLEDIGE VERSIE!!
1. Inleiding
1.1 Doel
Het doel van dit experiment is om bio ethanol te produceren. Dit word gedaan door aardappel
zetmeel met behulp van bacteriële amylase en bacteriële amyloglucosidase om te zetten in glucose.
Deze glucose word vervolgens met behulp van bakkersgist omgezet in ethanol.
