lOMoAR cPSD| 30867399
Clinical Chemistry - Bishop
Review Questions WITH Answers
, lOMoAR cPSD| 30867399
CLINICAL CHEMISTRY REVIEW QUESTIONS – BISHOP
ANALYTIC TECHNIQUES e. Charge used to separate solutes instead of size
1. Which of the following is not necessary for 9. Which of the following is not an advantage of
obtaining CE?
the spectrum of a compound from 190 to 500 a. Multiple samples can be assayed
nm? simultaneously
a. Tungsten light source on one injection
b. Deuterium light source b. Very small sample size
c. Double-beam spectrophotometer c. Rapid analysis
d. Quartz cuvettes d. Use of traditional detectors
e. Photomultiplier e. Cations, neutrals, and anions move in the same
direction at different rates
2. Stray light in a spectrophotometer places limits
on 10. Tandem mass spectrometers
a. Upper range of linearity a. Are two mass spectrometers placed in series
b. Sensitivity with each other
c. Photometric accuracy below 0.1 absorbance b. Are two mass spectrometers placed in parallel
units with each other
d. Ability to measure in the UV range c. Require use of a gas chromatograph
e. Use of a grating monochromator d. Require use of an electrospray interface
e. Do not require an ionization source
3. Which of the following light sources is used in
atomic absorption spectrophotometry? 11. Which of the following is false concerning the
a. Hollow-cathode lamp principles of point-of-care testing devices?
b. Xenon arc lamp a. Devices do not require quality control testing.
c. Tungsten light b. They use principles that are identical to
d. Deuterium lamp laboratory- based instrumentation.
e. Laser c. Biosensors have enabled miniaturization
particularly amendable for point-of-care testing.
4. Which of the following is true concerning d. Onboard microcomputers control instrument
fluorometry? functions and data reduction.
a. Fluorescence is an inherently more sensitive e. Whole blood analysis is the preferred
technique than absorption. specimen.
b. Emission wavelengths are always set at lower
wavelengths than excitation. 12. Which is the most sensitive detector for
c. The detector is always placed at right angles to spectrophotometry?
the excitation beam. a. Photomultiplier
d. All compounds undergo fluorescence. b. Phototube
e. Fluorometers require special detectors. c. Electron multiplier
d. Photodiode array
5. Which of the following techniques has the e. All are equally sensitive
highest
potential sensitivity? 13. Which of the following is Beer’s law?
a. Chemiluminescence d. Size used to separate solutes instead of charge
b. Fluorescence
c. Turbidimetry
d. Nephelometry
e. Phosphorescence
6. Which electrochemical assay measures current
at
fixed potential?
a. Amperometry
b. Anodic stripping voltammetry
c. Coulometry
d. Analysis with ISEs
e. Electrophoresis
7. Which of the following refers to the movement
of
buffer ions and solvent relative to the fixed
support?
a. Electroendosmosis
b. Isoelectric focusing
c. Iontophoresis
d. Zone electrophoresis
e. Plasmapheresis
, lOMoAR cPSD| 30867399
CLINICAL CHEMISTRY REVIEW QUESTIONS – BISHOP
a. A = e × b c
b. %T = I/I0 × 100
c. E = hν
d. e = ΔpH × 0.59 V
e. Osmolality = j × n × C
14. Which of the following correctly
ranks electromagnetic
radiation from low energy to high energy?
a. Microwaves, infrared, visible, UV, x-
rays,gamma, cosmic
b. Cosmic, gamma, x-rays, UV, visible,
infrared, microwaves
c. UV, visible, infrared, microwaves, x-
rays,cosmic, gamma
d. UV, visible, infrared, cosmic,
gamma, microwaves, x-rays
e. Visible, UV, infrared, cosmic,
gamma, microwaves, x-rays
15. What is the purpose of the chopper in
anatomic
absorption spectrophotometer?
a. Correct for the amount of light emitted by
the flame
b. Correct for the fluctuating intensity of the
lightsource
c. Correct for the fluctuating sensitivity of
the detector
d. Correct for differences in the aspiration rate
ofthe sample
Clinical Chemistry - Bishop
Review Questions WITH Answers
, lOMoAR cPSD| 30867399
CLINICAL CHEMISTRY REVIEW QUESTIONS – BISHOP
ANALYTIC TECHNIQUES e. Charge used to separate solutes instead of size
1. Which of the following is not necessary for 9. Which of the following is not an advantage of
obtaining CE?
the spectrum of a compound from 190 to 500 a. Multiple samples can be assayed
nm? simultaneously
a. Tungsten light source on one injection
b. Deuterium light source b. Very small sample size
c. Double-beam spectrophotometer c. Rapid analysis
d. Quartz cuvettes d. Use of traditional detectors
e. Photomultiplier e. Cations, neutrals, and anions move in the same
direction at different rates
2. Stray light in a spectrophotometer places limits
on 10. Tandem mass spectrometers
a. Upper range of linearity a. Are two mass spectrometers placed in series
b. Sensitivity with each other
c. Photometric accuracy below 0.1 absorbance b. Are two mass spectrometers placed in parallel
units with each other
d. Ability to measure in the UV range c. Require use of a gas chromatograph
e. Use of a grating monochromator d. Require use of an electrospray interface
e. Do not require an ionization source
3. Which of the following light sources is used in
atomic absorption spectrophotometry? 11. Which of the following is false concerning the
a. Hollow-cathode lamp principles of point-of-care testing devices?
b. Xenon arc lamp a. Devices do not require quality control testing.
c. Tungsten light b. They use principles that are identical to
d. Deuterium lamp laboratory- based instrumentation.
e. Laser c. Biosensors have enabled miniaturization
particularly amendable for point-of-care testing.
4. Which of the following is true concerning d. Onboard microcomputers control instrument
fluorometry? functions and data reduction.
a. Fluorescence is an inherently more sensitive e. Whole blood analysis is the preferred
technique than absorption. specimen.
b. Emission wavelengths are always set at lower
wavelengths than excitation. 12. Which is the most sensitive detector for
c. The detector is always placed at right angles to spectrophotometry?
the excitation beam. a. Photomultiplier
d. All compounds undergo fluorescence. b. Phototube
e. Fluorometers require special detectors. c. Electron multiplier
d. Photodiode array
5. Which of the following techniques has the e. All are equally sensitive
highest
potential sensitivity? 13. Which of the following is Beer’s law?
a. Chemiluminescence d. Size used to separate solutes instead of charge
b. Fluorescence
c. Turbidimetry
d. Nephelometry
e. Phosphorescence
6. Which electrochemical assay measures current
at
fixed potential?
a. Amperometry
b. Anodic stripping voltammetry
c. Coulometry
d. Analysis with ISEs
e. Electrophoresis
7. Which of the following refers to the movement
of
buffer ions and solvent relative to the fixed
support?
a. Electroendosmosis
b. Isoelectric focusing
c. Iontophoresis
d. Zone electrophoresis
e. Plasmapheresis
, lOMoAR cPSD| 30867399
CLINICAL CHEMISTRY REVIEW QUESTIONS – BISHOP
a. A = e × b c
b. %T = I/I0 × 100
c. E = hν
d. e = ΔpH × 0.59 V
e. Osmolality = j × n × C
14. Which of the following correctly
ranks electromagnetic
radiation from low energy to high energy?
a. Microwaves, infrared, visible, UV, x-
rays,gamma, cosmic
b. Cosmic, gamma, x-rays, UV, visible,
infrared, microwaves
c. UV, visible, infrared, microwaves, x-
rays,cosmic, gamma
d. UV, visible, infrared, cosmic,
gamma, microwaves, x-rays
e. Visible, UV, infrared, cosmic,
gamma, microwaves, x-rays
15. What is the purpose of the chopper in
anatomic
absorption spectrophotometer?
a. Correct for the amount of light emitted by
the flame
b. Correct for the fluctuating intensity of the
lightsource
c. Correct for the fluctuating sensitivity of
the detector
d. Correct for differences in the aspiration rate
ofthe sample